Microplastic pollution in aquafeed of diverse aquaculture animals.

Microplastics have emerged as pervasive contaminants, and determining their occurrence in aquafeed is key for evaluating their risks to farmed animals and, by extension, humans. However, knowledge about microplastic in aquafeed is still limited. Herein, we determined microplastic characteristics in aquafeed for five important aquaculture animals with different feeding habits. Aquafeed samples were collected for spotted sea bass, shrimp, grass carp, Tilapia, and frogs from main companies in China. The samples were digested using chemical digestion, and the residuals were subjected to a density separation. Microplastics were identified under the microscope and characterized by their shape, color, size, and polymer type. The results showed that microplastics are highly abundant in the feed of frogs, followed by spotted sea bass, Tilapia, grass carp, and shrimp. We found that feed size contributes to the total microplastic abundance in the feed. Further, microplastics were mainly in microfiber form, and the dominant polymer type was propylene, suggesting that packaging and processing are the main sources of pollution. Additionally, the most abundant size of microplastics was 100-1000 μm. Calculating microplastic ingestion risk, the spotted sea bass had the greatest recorded risk of microplastic ingestion, followed by grass carp, frogs, Tilapia, and shrimp. This study lays a foundational step toward understanding microplastic effects on aquaculture animals and calls for further environmentally relevant laboratory experiments to assess the risk of microplastic ingestion on animals and potential transfer to humans.