Evidence map›Paper›PMID 39294588›Full record

ArticleBMC microbiology2024

Occurrence rate and species and subtypes of Cryptosporidium spp. in pet dogs in Yunnan Province, China.

Jinhua Jian, Aiqin Liu, Yaming Yang, Xiaoxue Peng, Lan Yao, Benfu Li, Jinrong Zi, Jianping Cao, Yujuan Shen

Abstract read
In one paragraph

Article in BMC microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Molecular detection ofFrontiers in parasitology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jinhua JianNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, National Center for International Research on Tropical Diseases, Shanghai, 200025, China.
Aiqin LiuDepartment of Parasitology, Harbin Medical University, Harbin, 150081, China.
Yaming YangDepartment of Helminth, Yunnan Institute of Parasitic Diseases, Puer, 655099, China.
Xiaoxue PengNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, National Center for International Research on Tropical Diseases, Shanghai, 200025, China.
Lan YaoDepartment of Parasitology, Harbin Medical University, Harbin, 150081, China.
Benfu LiDepartment of Helminth, Yunnan Institute of Parasitic Diseases, Puer, 655099, China.
Jinrong ZiDepartment of Helminth, Yunnan Institute of Parasitic Diseases, Puer, 655099, China.
Jianping CaoNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, National Center for International Research on Tropical Diseases, Shanghai, 200025, China.
Yujuan ShenNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, National Center for International Research on Tropical Diseases, Shanghai, 200025, China. shenyj@nipd.chinacdc.cn.

Funding

National Nature Science Foundation of China Nos. 82372283 and 82072307Three-Year Initiative Plan for Strengthening Public Health System Construction in Shanghai (2023-2025) Key Discipline Project Nos. GWVI-11.1-12 and GWVI-11.1-09
6 · The paper itself

Abstract

backgroundCryptosporidium spp. is a ubiquitous, globally distributed intestinal protozoan infecting humans and at least 260 animal hosts. Due to close human contact with pet dogs and identification of zoonotic Cryptosporidium species and subtypes in these animals, dog health is not only a veterinarian issue but also a public health issue. This study aimed to understand occurrence and genetic characterization at both genotype and subtype levels in pet dogs in Yunnan Province, China.

resultsA total of 589 fresh fecal specimens were collected from adult pet dogs in the rural areas of eight cities/autonomous prefectures of Yunnan Province, China. 16 fecal specimens were positive for Cryptosporidium spp. by polymerase chain reaction (PCR) amplification and sequence analysis of the small subunit ribosomal RNA (SSU rRNA) gene, with an average occurrence rate of 2.7% (16/589) being observed. Three zoonotic Cryptosporidium species were identified: C. parvum (n = 7), C. suis (n = 5) and C. canis (n = 4). At the 60-kDa glycoprotein (gp60) locus, only three C. parvum and two C. canis specimens were successfully amplified and sequenced, with subtype IIaA17G2R1 (n = 3) and subtypes XXa4 (n = 1) and XXa5 (n = 1) being identified, respectively.

conclusionsThe present finding of three zoonotic Cryptosporidium species in dogs implied that dogs infected with Cryptosporidium spp. may pose a threat to human health. C. suis was identified in dogs in this study for the first time, expanding the host range of this species. Identification of C. parvum subtype IIaA17G2R1 and C. canis subtypes XXa4 and XXa5 will be helpful to explore the source attribution of infection/contamination and assess the transmission dynamics of C. parvum and C. canis in the investigated areas in the future.

Indexed as

CryptosporidiosisCryptosporidiumDog DiseasesFecesGenotypePetsPhylogenyAnimalsChinaDNA, ProtozoanDNA, RibosomalDogsMolecular Sequence DataPolymerase Chain ReactionSequence Analysis, DNADNA, ProtozoanDNA, RibosomalCryptosporidiumDogsGenotypingSubtypingZoonotic transmission

Identifiers

PMID39294588
PMCPMC11411820

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.