ArticleMicrobial ecology2024
Gardnerella vaginalis, Fannyhessea vaginae, and Prevotella bivia Strongly Influence Each Other's Transcriptome in Triple-Species Biofilms.
Article in Microbial ecology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Bridging the gap:Gut microbes · 2026Review
- Genome-scale metabolic modelling identifies vaginal microbiome members as potential probiotics.Nature microbiology · 2026Article
- Dynamics of Extracellular Traps in Vaginal Dysbiosis Associated withInternational journal of molecular sciences · 2026Article
- The role of Traditional Chinese Medicine in the management of liver disease: targeting gut microbiome.Chinese medicine · 2026Review
- Phage therapy for treatment of bacterial vaginosis.Archives of microbiology · 2026Review
- Lactobacillus-Dominated Cervical Microbiota Revealed by Long-Read 16S rRNA Sequencing: A Greek Pilot Study.Genes · 2025Article
- Mixed Infections in the Female Lower Genital Tract: Unlocking the Current Landscape and Future Directions.Current medical science · 2025Review
- Metagenomic analysis reveals the novel role of vaginal Lactobacillus iners in Chinese healthy pregnant women.NPJ biofilms and microbiomes · 2025Article
- Vaginal Dysbiosis in Infertility: A Comparative Analysis Between Women with Primary and Secondary Infertility.Microorganisms · 2025Article
- Adaptation of key bacterial vaginosis-associated bacteria to a medium simulating genital tract secretions: a transcriptomic analysis.Frontiers in genetics · 2025Article
- Vaginal microbiota: different roles of lactobacilli and community instability in chronic vulvovaginal discomfort.Frontiers in cellular and infection microbiology · 2025Article
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Authors and funding
5 authors.
Funding
Abstract
Bacterial vaginosis (BV), the most common vaginal infection worldwide, is characterized by the development of a polymicrobial biofilm on the vaginal epithelium. While Gardnerella spp. have been shown to have a prominent role in BV, little is known regarding how other species can influence BV development. Thus, we aimed to study the transcriptome of Gardnerella vaginalis, Fannyhessea vaginae, and Prevotella bivia, when growing in triple-species biofilms. Single and triple-species biofilms were formed in vitro, and RNA was extracted and sent for sequencing. cDNA libraries were prepared and sequenced. Quantitative PCR analysis (qPCR) was performed on the triple-species biofilms to evaluate the biofilm composition. The qPCR results revealed that the triple-species biofilms were mainly composed by G. vaginalis and P. bivia was the species with the lowest percentage. The RNA-sequencing analysis revealed a total of 432, 126, and 39 differentially expressed genes for G. vaginalis, F. vaginae, and P. bivia, respectively, when growing together. Gene ontology enrichment of G. vaginalis downregulated genes revealed several functions associated with metabolism, indicating a low metabolic activity of G. vaginalis when growing in polymicrobial biofilms. This work highlighted that the presence of 3 different BV-associated bacteria in the biofilm influenced each other's transcriptome and provided insight into the molecular mechanisms that enhanced the virulence potential of polymicrobial consortia. These findings will contribute to understand the development of incident BV and the interactions occurring within the biofilm.
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