Evidence map›Paper›PMID 39293721›Full record

ArticleRadiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology2024

A multi-institutional study to investigate the sparing effect after whole brain electron FLASH in mice: Reproducibility and temporal evolution of functional, electrophysiological, and neurogenic endpoints.

Olivia G G Drayson, Stavros Melemenidis, Nikita Katila, Vignesh Viswanathan, Enikö A Kramár, Richard Zhang, Rachel Kim, Ning Ru, Benoit Petit, Suparna Dutt and 15 more

Abstract readMulticenter Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Article
  3. Volumetric FLASH dosimetryPhysics in medicine and biology · 2026
    Article
  4. Review
  5. Article
  6. Microenvironmental remodeling in FLASH radiotherapy.Frontiers in cell and developmental biology · 2026
    Review
  7. Review
  8. Review
  9. Review
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

25 authors.

Olivia G G DraysonDepartment of Radiation Oncology, University of California, Irvine, CA 92697, USA.
Stavros MelemenidisDepartment of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Nikita KatilaDepartment of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Vignesh ViswanathanDepartment of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Enikö A KramárDepartment of Neurobiology and Behavior, University of California, Irvine, CA 92697, USA.
Richard ZhangDepartment of Radiation Oncology, University of California, Irvine, CA 92697, USA.
Rachel KimDepartment of Radiation Oncology, University of California, Irvine, CA 92697, USA.
Ning RuDepartment of Radiation Oncology, University of California, Irvine, CA 92697, USA.
Benoit PetitLaboratory of Radiation Oncology, Department of Radiation Oncology, Lausanne University Hospital and University of Lausanne, Switzerland.
Suparna DuttDepartment of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Rakesh ManjappaDepartment of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA.
M Ramish AshrafDepartment of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Brianna LauDepartment of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Luis SotoDepartment of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Lawrie SkinnerDepartment of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Amu S YuDepartment of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Murat SurucuDepartment of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Peter G MaximDepartment of Radiation Oncology, University of California, Irvine, CA 92697, USA.
Paola Zebadua-BallasterosLaboratory of Radiation Oncology, Department of Radiation Oncology, Lausanne University Hospital and University of Lausanne, Switzerland; Laboratorio de Fisica Medica, Instituto Nacional de Neurología y Neurocirugía MVS, México City 14269, Mexico.
Marcelo A WoodDepartment of Neurobiology and Behavior, University of California, Irvine, CA 92697, USA.
Pierre Montay-GruelLaboratory of Radiation Oncology, Department of Radiation Oncology, Lausanne University Hospital and University of Lausanne, Switzerland; Radiation Oncology Department, Iridium Netwerk, Wilrijk, Antwerp, Belgium; Antwerp Research in Radiation Oncology (AReRO), Centre for Oncological Research (CORE), University of Antwerp, Antwerp, Belgium.
Janet E BaulchDepartment of Radiation Oncology, University of California, Irvine, CA 92697, USA.
Marie-Catherine VozeninLaboratory of Radiation Oncology, Department of Radiation Oncology, Lausanne University Hospital and University of Lausanne, Switzerland. Electronic address: marie-catherine.vozenin@hcuge.ch.
Billy W LooDepartment of Radiation Oncology, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: bwloo@stanford.edu.
Charles L LimoliDepartment of Radiation Oncology, University of California, Irvine, CA 92697, USA. Electronic address: climoli@uci.edu.

Funding

Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Melanie Funes · 1994 to 2026
$57.9M
Project 4: Mechanistic Basics of FLASH Effect: Role of O2P01CA244091 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI LIMOLI, CHARLES · 2020 to 2024
$9.6M
NCI NIH HHS P01 CA244091NCI NIH HHS P30 CA062203
6 · The paper itself

Abstract

background and purposeUltra-high dose-rate radiotherapy (FLASH) has been shown to mitigate normal tissue toxicities associated with conventional dose rate radiotherapy (CONV) without compromising tumor killing in preclinical models. A prominent challenge in preclinical radiation research, including FLASH, is validating both the physical dosimetry and the biological effects across multiple institutions. MATERIALS AND

methodsWe previously demonstrated dosimetric reproducibility of two different electron FLASH devices at separate institutions using standardized phantoms and dosimeters. In this study, tumor-free adult female mice were given 10 Gy whole brain FLASH and CONV irradiation at both institutions and evaluated for the reproducibility and temporal evolution of multiple neurobiological endpoints.

resultsFLASH sparing of behavioral performance on novel object recognition (4 months post-irradiation) and of electrophysiologic long-term potentiation (LTP, 5 months post-irradiation) was reproduced between institutions. Differences between FLASH and CONV on the endpoints of hippocampal neurogenesis (Sox2, doublecortin), neuroinflammation (microglial activation), and electrophysiology (LTP) were not observed at early times (48 h to 2 weeks), but recovery of immature neurons by 3 weeks was greater with FLASH.

conclusionIn summary, we demonstrated reproducible FLASH sparing effects on the brain between two different beams at two different institutions with validated dosimetry. FLASH sparing effects on the endpoints evaluated manifested at later but not the earliest time points.

Indexed as

NeurogenesisAnimalsBrainFemaleHippocampusLong-Term PotentiationMiceMice, Inbred C57BLRadiotherapy DosageReproducibility of ResultsElectrophysiologyFLASHIntercomparisonNeurobehaviorNeurogenesisNeuroinflammationRadiotherapy

Identifiers

PMID39293721
PMCPMC11588524

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