Evidence map›Paper›PMID 39293710›Full record

ArticleMetabolic engineering2024

Architecting a transcriptional repressor-based genetic inverter for tryptophan derived pathway regulation in Escherichia coli.

Xinyu Gong, Yuxi Teng, Jianli Zhang, Qi Gan, Ming Song, Ameen Alaraj, Peter Kner, Yajun Yan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Metabolic engineering, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Engineering a heme-dependent tryptophan hydroxylase pathway inSynthetic and systems biotechnology · 2026
    Article
  2. Engineering theACS synthetic biology · 2026
    Article
  3. Review
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xinyu GongSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, GA, 30602, USA.
Yuxi TengSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, GA, 30602, USA.
Jianli ZhangSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, GA, 30602, USA.
Qi GanSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, GA, 30602, USA.
Ming SongSchool of Electrical and Computer Engineering, College of Engineering, The University of Georgia, Athens, GA, 30602, USA.
Ameen AlarajSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, GA, 30602, USA.
Peter KnerSchool of Electrical and Computer Engineering, College of Engineering, The University of Georgia, Athens, GA, 30602, USA.
Yajun YanSchool of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, GA, 30602, USA. Electronic address: yajunyan@uga.edu.

Funding

Engineering Dynamic Control of Natural Product Biosynthesis in BacteriaR35GM128620 · NIGMS · UNIVERSITY OF GEORGIA · PI Yajun Yan · 2018 to 2026
$3.0M
NIGMS NIH HHS R35 GM128620
6 · The paper itself

Abstract

Efficient microbial cell factories require intricate and precise metabolic regulations for optimized production, which can be significantly aided by implementing regulatory genetic circuits with versatile functions. However, constructing functionally diverse genetic circuits in host strains is challenging. Especially, functional diversification based on transcriptional repressors has been rarely explored due to the difficulty in inverting their repression properties. To address this, we proposed a design logic to create transcriptional repressor-based genetic inverters for functional enrichment. As proof of concept, a tryptophan-inducible genetic inverter was constructed by integrating two sets of transcriptional repressors, PtrpO1-TrpR1 and PtetO1-TetR. In this genetic inverter, the repression of TetR towards PtetO1 could be alleviated by the tryptophan-TrpR1 complex in the presence of tryptophan, leading to the activated output. Subsequently, we optimized the dynamic performance of the inverter and constructed tryptophan-triggered dynamic activation systems. Further coupling of the original repression function of PtrpO1-TrpR1 with inverter variants realized the tryptophan-triggered bifunctional regulation system. Finally, the dynamic regulation systems enabled tryptophan production monitoring. These systems also remarkably increased the titers of the tryptophan derivatives tryptamine and violacein by 2.0-fold and 7.4-fold, respectively. The successful design and application of the genetic inverter enhanced the applicability of transcriptional repressors.

Indexed as

Escherichia coliEscherichia coli ProteinsRepressor ProteinsTryptophanGene Expression Regulation, BacterialMetabolic EngineeringEscherichia coli ProteinsRepressor ProteinsTRPR protein, E coliTryptophanDynamic regulationGenetic inverterTetRTranscriptional repressorTrpRTryptamineViolacein

Identifiers

PMID39293710
PMCPMC11611649

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.