Evidence map›Paper›PMID 39289934›Full record

ArticleCurrent pharmaceutical biotechnology2025

Ferulic Acid Regulates GSDMD through the ROS/JNK/Bax Mitochondrial Apoptosis Pathway to Induce Pyroptosis in Lung Cancer.

Xingchen Liu, Xin Zhang, Lida Mi, Hailin Zhang, Woda Shi

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Article in Current pharmaceutical biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

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6citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Xingchen LiuDepartment of Cardiothoracic Surgery, Affiliated Hospital 6 of Nantong University, The Yancheng School of Clinical Medicine of Nanjing Medical University, Yancheng Third People's Hospital, Yancheng, Jiangsu, 224000, China.
Xin ZhangDepartment of Cardiothoracic Surgery, Affiliated Hospital 6 of Nantong University, The Yancheng School of Clinical Medicine of Nanjing Medical University, Yancheng Third People's Hospital, Yancheng, Jiangsu, 224000, China.
Lida MiDepartment of Cardiothoracic Surgery, Affiliated Hospital 6 of Nantong University, The Yancheng School of Clinical Medicine of Nanjing Medical University, Yancheng Third People's Hospital, Yancheng, Jiangsu, 224000, China.
Hailin ZhangDepartment of Pneumology, Affiliated Hospital 6 of Nantong University, The Yancheng School of Clinical Medicine of Nanjing Medical University, Yancheng Third People's Hospital, Yancheng, Jiangsu, 224000, China.
Woda ShiDepartment of Cardiothoracic Surgery, Affiliated Hospital 6 of Nantong University, The Yancheng School of Clinical Medicine of Nanjing Medical University, Yancheng Third People's Hospital, Yancheng, Jiangsu, 224000, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTo improve the prognosis outcome of lung cancer patients, more investigations are still needed. Previous reports have demonstrated the function of Ferulic Acid (FA) in lung cancer; thus, we have attempted to probe more molecular mechanisms underlying FA application in lung cancer.

methodsCCK8 and colony formation experiments have been employed to explore cell viability and proliferation. Cell apoptosis was evaluated through flow cytometry. Cell morphology was observed with a microscope. MMP was assessed by JC-1 and LDH activity was evaluated by relative kit. Western blot assays were performed to examine the expression levels of GSDMD, GSDMD-N, caspase family proteins, and ROS/JNK/Bax mitochondrial apoptosis pathway downstream proteins. Flow cytometry analysis also measured the level of ROS. Tissues from animal models were taken for IHC analysis of C-caspase-1.

resultsFA was found to inhibit proliferation, change cell morphology, decrease MMP, and enhance LDH activity, suggesting its ability to induce pyroptosis of lung cancer cells. Both caspase-1 and GSDMD were found to be involved in the pyroptosis of lung cancer cells treated with FA, and caspase-1 mediated GSDMD. Moreover, FA was validated to regulate pyroptosis by ROS/JNK/Bax mitochondrial apoptosis pathway

conclusionIn summary, FA regulates GSDMD through ROS/JNK/Bax mitochondrial apoptosis pathway to induce pyroptosis in lung cancer cells, which may offer a theoretical basis for pyroptosis in the occurrence of lung cancer.

Indexed as

Coumaric AcidsIntracellular Signaling Peptides and ProteinsLung NeoplasmsPhosphate-Binding ProteinsPyroptosisA549 CellsAnimalsApoptosisbcl-2-Associated X ProteinCell Line, TumorCell ProliferationGasderminsHumansMaleMAP Kinase Signaling SystemMiceBAX protein, humanbcl-2-Associated X ProteinCoumaric Acidsferulic acidGasderminsGSDMD protein, humanIntracellular Signaling Peptides and ProteinsPhosphate-Binding ProteinsReactive Oxygen Speciescaspase-1ferulic acidGSDMDLung cancerpyroptosis.ROS/JNK/Bax mitochondrial apoptosis pathway

Identifiers

PMID39289934

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.