Evidence map›Paper›PMID 39289419›Full record

ArticleScientific reports2024

Anastomotic leak occurs independently from microbiota shifts associated with surgical bowel preparation.

Sonja Boatman, Mohammad Haneef Khan, Nirupa Ganesan, Harika Nalluri-Butz, Julia Kohn, Alexander Troester, Zachary Ziegert, Robert Madoff, Wolfgang B Gaertner, Cyrus Jahansouz and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Modulation of the gut microbiota as a novel strategy to prevent anastomotic leak after colorectal surgery: Systematic scoping review.Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland · 2026
    Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Sonja BoatmanBasic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA.
Mohammad Haneef KhanBasic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA.
Nirupa GanesanBasic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA.
Harika Nalluri-ButzBasic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA.
Julia KohnBasic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA.
Alexander TroesterBasic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA.
Zachary ZiegertBasic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA.
Robert MadoffBasic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA.
Wolfgang B GaertnerBasic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA.
Cyrus JahansouzBasic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA. jahan023@umn.edu.
Christopher StaleyBasic and Translational Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, MCC 195, Minneapolis, MN, 55455, USA. cmstaley@umn.edu.

Funding

American Society of Colon and Rectal Surgeons CDA-020
6 · The paper itself

Abstract

Following bowel surgery, infectious complications, including anastomotic leak (AL), remain major sources of morbidity and mortality. Bowel preparation is often administered with the assumption that gut decontamination reduces post-surgical complications. In this study, we tested this hypothesis using a murine model of colon surgery. The mice were fed either regular chow or a high-fat, high-sugar Western diet. The day before surgery, the mice received one of four interventions: water (control), mechanical bowel preparation (MBP), oral antibiotics (OA), or both MBP and OA. We found no differences in the rates of AL among the experimental groups, and diet did not appear to affect the outcomes. Exploratory analyses showed changes in the gut microbiome consistent with the different treatments, but investigations of fecal short-chain fatty acids and RNA sequencing of colonic tissue did not reveal specific effects of the treatments or the presence of AL. However, we did identify bacterial genera that may be causally associated with AL and developed a predictive index from stool samples as a marker for the presence of AL. Future research is needed to identify and validate a microbial predictive tool and to uncover the microbial-driven mechanisms that lead to AL.

Indexed as

Anastomotic LeakGastrointestinal MicrobiomeAnimalsAnti-Bacterial AgentsColonDisease Models, AnimalFatty Acids, VolatileFecesMaleMiceMice, Inbred C57BLAnti-Bacterial AgentsFatty Acids, VolatileAnastomotic leakColorectal surgeryMicrobiomeMouse modelWestern diet

Identifiers

PMID39289419
PMCPMC11408509

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.