Evidence map›Paper›PMID 39286224›Full record

ArticleHeliyon2024

Development and evaluation of a duplex real-time multienzyme isothermal rapid amplification assay for the detection of hypervirulent

Zhixiong Duan, Shan Wang, Niqi Xie, Junying Zhao, Jian Dong, Jin Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Zhixiong DuanDepartment of Laboratory Medicine, The Affiliated Dazu's Hospital of Chongqing Medical University, Chongqing, China.
Shan WangDepartment of Laboratory Medicine, The Affiliated Dazu's Hospital of Chongqing Medical University, Chongqing, China.
Niqi XieDepartment of Laboratory Medicine, The Affiliated Dazu's Hospital of Chongqing Medical University, Chongqing, China.
Junying ZhaoDepartment of Laboratory Medicine, The Affiliated Dazu's Hospital of Chongqing Medical University, Chongqing, China.
Jian DongDepartment of Laboratory Medicine, The Affiliated Dazu's Hospital of Chongqing Medical University, Chongqing, China.
Jin LiDepartment of Laboratory Medicine, The Affiliated Dazu's Hospital of Chongqing Medical University, Chongqing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objectives: Our objective was to establish a rapid and precise method for detecting hypervirulent Methods: The research comprised two phases: an initial pilot study to establish the methodology and a clinical validation study to assess its effectiveness. In the pilot phase, we designed specific primers and probes targeting the hvKP Results: The duplex real-time MIRA assay demonstrated no cross-reactivity with other strains. Sensitivity experiments confirmed that the assay had a detection limit as low as 8 × 10 Conclusions: These findings confirm that the duplex real-time MIRA assay is a fast, straightforward, and dependable method for detecting hvKP.

Indexed as

Duplex real-time MIRADuplex real-time PCRHypervirulent Klebsiella pneumoniaepg344 and incA genes

Identifiers

PMID39286224
PMCPMC11402988

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.