Evidence map›Paper›PMID 39285500›Full record

ArticleStem cell research & therapy2024

NQO1 promotes osteogenesis and suppresses angiogenesis in DPSCs via MAPK pathway modulation.

Wanqing Wang, Haoqing Yang, Zhipeng Fan, Ruitang Shi

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Article in Stem cell research & therapy, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Wanqing WangBeijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing, 100050, China.
Haoqing YangBeijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing, 100050, China.
Zhipeng FanBeijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing, 100050, China. zpfan@ccmu.edu.cn.ORCID 0000-0003-1662-9420
Ruitang ShiDepartment of Endodontics, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing, 100050, China. shiruitang@126.com.

Funding

Chinese Academy of Medical Sciences Initiative for Innovative Medicine 2019-I2M-5-031Key Technologies Research and Development Program 2022YFA1104401National Natural Science Foundation of China 82071071National Natural Science Foundation of China 82130028
6 · The paper itself

Abstract

backgroundInfluence on stem cells' angiogenesis and osteogenesis of NAD(P)H Quinone Dehydrogenase 1(NQO1) has been established, but its impact on dental pulp stem cells (DPSCs) is unexplored. An important strategy for the treatment of arteriosclerosis is to inhibit calcium deposition and to promote vascular repair and angiogenesis. This study investigated the function and mechanism of NQO1 on angiogenesis and osteogenesis of DPSCs, so as to provide a new ideal for the treatment of arteriosclerosis.

methodsCo-culture of human DPSCs and human umbilical vein endothelial cells (HUVECs) was used to detect the angiogenesis ability. Alkaline phosphatase (ALP) activity, alizarin red staining (ARS), and transplantation of HA/tricalcium phosphate with DPSCs were used to detect osteogenesis.

resultsNQO1 suppressed in vitro tubule formation, migration, chemotaxis, and in vivo angiogenesis, as evidenced by reduced CD31 expression. It also enhanced ALP activity, ARS, DSPP expression and osteogenesis and boosted mitochondrial function in DPSCs. CoQ10, an electron transport chain activator, counteracted the effects of NQO1 knockdown on these processes. Additionally, NQO1 downregulated MAPK signaling, which was reversed by CoQ10 supplementation in DPSCs-NQO1sh.

conclusionsNQO1 inhibited angiogenesis and promoted the osteogenesis of DPSCs by suppressing MAPK signaling pathways and enhancing mitochondrial respiration.

Indexed as

Dental PulpHuman Umbilical Vein Endothelial CellsMAP Kinase Signaling SystemNAD(P)H Dehydrogenase (Quinone)Neovascularization, PhysiologicOsteogenesisAngiogenesisAnimalsCell DifferentiationCells, CulturedCoculture TechniquesHumansStem CellsUbiquinoneNAD(P)H Dehydrogenase (Quinone)NQO1 protein, humanUbiquinoneAngiogenesisDPSCMitochondrial respirationNQO1Osteogenesis

Identifiers

PMID39285500
PMCPMC11406740

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.