Evidence map›Paper›PMID 39285497›Full record

ArticleBMC research notes2024

Aqueous extraction of formalin-fixed paraffin-embedded tissue and detection of prion disease using real-time quaking-induced conversion.

Eric M Nicholson, Justin J Greenlee, Soyoun Hwang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Eric M NicholsonVirus and Prion Research Unit, National Animal Disease Center, USDA-ARS, 1920 Dayton Ave., Ames, IA, 50010, USA. Eric.Nicholson@usda.gov.
Justin J GreenleeVirus and Prion Research Unit, National Animal Disease Center, USDA-ARS, 1920 Dayton Ave., Ames, IA, 50010, USA.
Soyoun HwangVirus and Prion Research Unit, National Animal Disease Center, USDA-ARS, 1920 Dayton Ave., Ames, IA, 50010, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe goal of the research presented here is to determine if methods previously developed for the aqueous extraction of PrP

resultsWe found complete agreement between the TSE status and the results from RT-QuIC seeded with the aqueous extract of FFPET samples. The method affords the diagnostic assessment TSE status by RT-QuIC of FFPET without the use of organic solvents that would otherwise create a mixed chemical-biological waste for disposal.

Indexed as

FormaldehydeParaffin EmbeddingPrion DiseasesPrPSc ProteinsTissue FixationAnimalsHumansMiceFormaldehydePrPSc ProteinsParaffin-embedded tissuePrionPrPRT-QuICSpongiform encephalopathyTransmissible spongiform encephalopathy

Identifiers

PMID39285497
PMCPMC11403835

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.