Evidence map›Paper›PMID 39285447›Full record

ArticleClinical epigenetics2024

Technical considerations for placental tissue processing and the subsequent impact on genome-wide DNA methylation analysis.

Kristen M Kocher, Julius Ngwa, Kushal J Kapse, Surajit Bhattacharya, Christopher Rossi, Emmanuele Delot, Adre duPlessis, Catherine Limperopoulos, Nickie Andescavage

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical epigenetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Kristen M KocherDeveloping Brain Institute, Center for Prenatal, Neonatal and Maternal Health Research, Children's Research Institute, Children's National Hospital, Washington, DC, USA.
Julius NgwaDeveloping Brain Institute, Center for Prenatal, Neonatal and Maternal Health Research, Children's Research Institute, Children's National Hospital, Washington, DC, USA.
Kushal J KapseDeveloping Brain Institute, Center for Prenatal, Neonatal and Maternal Health Research, Children's Research Institute, Children's National Hospital, Washington, DC, USA.
Surajit BhattacharyaCenter for Genetic Medicine Research, Children's Research Institute, Children's National Hospital, Washington, DC, USA.
Christopher RossiDepartment of Pathology and Laboratory Medicine, Children's National Hospital, Washington, DC, USA.
Emmanuele DelotCenter for Genetic Medicine Research, Children's Research Institute, Children's National Hospital, Washington, DC, USA.
Adre duPlessisDepartment of Pediatrics, The George Washington University, Washington, DC, USA.
Catherine LimperopoulosDeveloping Brain Institute, Center for Prenatal, Neonatal and Maternal Health Research, Children's Research Institute, Children's National Hospital, Washington, DC, USA.
Nickie AndescavageDeveloping Brain Institute, Center for Prenatal, Neonatal and Maternal Health Research, Children's Research Institute, Children's National Hospital, Washington, DC, USA.

Funding

Clinical and Translational Science Institute at Childrens NationalUL1TR001876 · NCATS · CHILDREN'S RESEARCH INSTITUTE · PI BELL, MICHAEL J · 2016 to 2021
$25.6M
Diversity Supplement: Clinical Pediatric Research Training for the Fetal-Neonatal BrainT32HD098066 · NICHD · CHILDREN'S RESEARCH INSTITUTE · PI DU PLESSIS, ADRE JACQUES, LIMPEROPOULOS, CATHERINE · 2020 to 2024
$1.7M
Eunice Kennedy Shriver National Institute of Child Health and Human Development T32HD098066NCATS NIH HHS UL1 TR001876NCATS NIH HHS UL1TR001876NICHD NIH HHS T32 HD098066
6 · The paper itself

Abstract

To assess the impact of postnatal processing on placental DNA methylation, array data from flash-frozen placental tissue was compared to perfluorocarbon-immersed and formalin-fixed paraffin-embedded placental tissue. We observed that tissue exposed to perfluorocarbon showed no significant DNA methylation differences when compared to unprocessed tissue, while formalin processing altered the quality and reliability of the data produced on the DNA methylation array platform. Placental DNA methylation allows for the study of gene-environment interactions that influence the fetal environment and development. Our study highlights that placental post-processing techniques must be considered in the evaluation and interpretation of epigenetic studies.

Indexed as

DNA MethylationPlacentaEpigenesis, GeneticEpigenomicsFemaleHumansParaffin EmbeddingPregnancyDNA methylationEpigenetic agingFFPEPlacenta

Identifiers

PMID39285447
PMCPMC11403961

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.