Evidence map›Paper›PMID 39282536›Full record

ArticleLe infezioni in medicina2024

Promoting molecular diagnostic equity: assessing in-house real-time PCR for

Rokaya Aitlhaj-Mhand, Aicha Qasmaoui, Bahija Bellaji, Chaimae Remz, Reda Charof, Rachid El Jaoudi, Hanaa Abdelmoumen, Amina Hançali, Hicham Oumzil

Abstract read
In one paragraph

Article in Le infezioni in medicina, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Development and Laboratory Evaluation of aInternational journal of molecular sciences · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Rokaya Aitlhaj-MhandVirology Department, National Institute of Hygiene, Rabat, Morocco.
Aicha QasmaouiEpidemic Diseases Laboratory, Medical Bacteriology Department, National Institute of Hygiene, Rabat, Morocco.
Bahija BellajiSTIs Reference Laboratory, Medical Bacteriology Department, National Institute of Hygiene, Rabat, Morocco.
Chaimae RemzVirology Department, National Institute of Hygiene, Rabat, Morocco.
Reda CharofEpidemic Diseases Laboratory, Medical Bacteriology Department, National Institute of Hygiene, Rabat, Morocco.
Rachid El JaoudiMedical Biotechnology Pedagogy and Research Unit, School of Medicine and Pharmacy, Mohammed V University in Rabat, Morocco.
Hanaa AbdelmoumenMicrobiology and Molecular Biology Team, Research Center for Plant and Microbial Biotechnology, Biodiversity and Environment, Faculty of Sciences, Mohammed V University in Rabat, Rabat, Morocco.
Amina HançaliSTIs Reference Laboratory, Medical Bacteriology Department, National Institute of Hygiene, Rabat, Morocco.
Hicham OumzilMedical Biotechnology Pedagogy and Research Unit, School of Medicine and Pharmacy, Mohammed V University in Rabat, Morocco.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objectives: Gonorrhea is a prevalent sexually transmitted infection among men who have sex with men (MSM). In Morocco, the basic laboratory diagnosis of Patients and methods: Samples from 245 MSM, recruited using a Respondent Driven Sampling, were collected and tested for NG infection using GeneXpert CT/NG assay (Cepheid, USA). An In-House real-time PCR technique targeting the pseudo gene porA was developed and used for a parallel investigation of the same infection. The reliability of the in-house RT-PCR was validated through tests of reproducibility, repeatability, limit of detection, and cross-reactivity with other bacteria. The intrinsic performance characteristics of the qRT-PCR were assessed, namely, the sensitivity, the specificity, the positive predictive value (PPV), and the negative predictive value (NPV). The GeneXpert CT/NG assay was adopted as a reference method. Results: For Conclusion: The in-house real-time PCR assay has high specificity and sensitivity, and it emerges as a promising approach for detecting

Indexed as

MoroccoNeisseria gonorrhoeaeporA pseudogenereal time PCR

Identifiers

PMID39282536
PMCPMC11392542

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.