Evidence map›Paper›PMID 39282370›Full record

ArticlebioRxiv : the preprint server for biology2024

Antigen specificity of clonally-enriched CD8+ T cells in multiple sclerosis.

Kristen Mittl, Fumie Hayashi, Ravi Dandekar, Ryan D Schubert, Josiah Gerdts, Lindsay Oshiro, Rita Loudermilk, Ariele Greenfield, Danillo G Augusto, Akshaya Ramesh and 21 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

31 authors.

Kristen MittlUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Fumie HayashiUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Ravi DandekarUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Ryan D SchubertUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.ORCID 0000-0002-3132-5831
Josiah GerdtsUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Lindsay OshiroUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Rita LoudermilkUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Ariele GreenfieldUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Danillo G AugustoUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Akshaya RameshUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Edwina TranUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Kaniskha KoshalUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Kerry KizerUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Joanna Dreux3T Biosciences, South San Francisco, CA, USA.
Alaina Cagalingan3T Biosciences, South San Francisco, CA, USA.
Florian Schustek3T Biosciences, South San Francisco, CA, USA.
Lena Flood3T Biosciences, South San Francisco, CA, USA.
Tamson Moore3T Biosciences, South San Francisco, CA, USA.
Lisa L Kirkemo3T Biosciences, South San Francisco, CA, USA.
Tiffany CooperUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Meagan HarmsUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Refujia GomezUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
University of California, San Francisco MS-EPIC Team
Leah Sibener3T Biosciences, South San Francisco, CA, USA.
Bruce A C CreeUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.ORCID 0000-0001-7689-2533
Stephen L HauserUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.ORCID 0000-0002-4932-4001
Jill A HollenbachUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Marvin Gee3T Biosciences, South San Francisco, CA, USA.
Michael R WilsonUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.ORCID 0000-0002-8705-5084
Scott S ZamvilUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.
Joseph J SabatinoUCSF Weill Institute for Neurosciences, Department of Neurology, University of California, San Francisco, CA, USA.ORCID 0000-0002-6804-7441

Funding

The Role of B cells in the Origin and Progression of Multiple SclerosisR35NS111644 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI STEPHEN L HAUSER · 2019 to 2026
$9.1M
The landscape of HLA mediated variation in health and immunityR01AI158861 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HOLLENBACH, JILL ALLISON · 2021 to 2025
$3.5M
Role of Natural Killer Cell Diversity in Multiple Sclerosis Risk and Disease CourseR01AI169070 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JILL Allison HOLLENBACH, Paul John Norman · 2022 to 2026
$3.0M
Disease relevance of CD20 expression on T cells in multiple sclerosis patientsR01NS092835 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HAUSER, STEPHEN L · 2015 to 2018
$1.7M
Detection of disease-relevant CD8+ T cells in Multiple SclerosisK08NS107619 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI SABATINO, JOSEPH JOHN · 2019 to 2023
$1.0M
A synthetic biosensor of immunologic synapse formation allowing multiplexed T cell antigen discovery for autoimmune neurologic disordersK08AI174061 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JOSIAH GERDTS · 2023 to 2026
$735k
Single cell RNA-Seq and clonal analysis of CD8+ T cells in MSR21AI142186 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ZAMVIL, SCOTT S · 2019 to 2020
$443k
NIAID NIH HHS K08 AI174061NIAID NIH HHS R01 AI158861NIAID NIH HHS R01 AI169070NIAID NIH HHS R21 AI142186NINDS NIH HHS K08 NS107619NINDS NIH HHS R01 NS092835NINDS NIH HHS R35 NS111644
6 · The paper itself

Abstract

CD8+ T cells are the dominant lymphocyte population in multiple sclerosis (MS) lesions where they are highly clonally expanded. The clonal identity, function, and antigen specificity of CD8+ T cells in MS are not well understood. Here we report a comprehensive single-cell RNA-seq and T cell receptor (TCR)-seq analysis of the cerebrospinal fluid (CSF) and blood from a cohort of treatment-naïve MS patients and control participants. A small subset of highly expanded and activated CD8+ T cells were enriched in the CSF in MS that displayed high activation, cytotoxicity and tissue-homing transcriptional profiles. Using a combination of unbiased and targeted antigen discovery approaches, MS-derived CD8+ T cell clonotypes recognizing Epstein-Barr virus (EBV) antigens and multiple novel mimotopes were identified. These findings shed vital insight into the role of CD8+ T cells in MS and pave the way towards disease biomarkers and therapeutic targets.

Identifiers

PMID39282370
PMCPMC11398516

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.