Evidence map›Paper›PMID 39282306›Full record

ArticlebioRxiv : the preprint server for biology2024

MLX phosphorylation stabilizes the ChREBP-MLX heterotetramer on tandem E-boxes to control carbohydrate and lipid metabolism.

Carla E Cadena Del Castillo, Onur Deniz, Femke van Geest, Lore Rosseels, Ingrid Stockmans, Marius Robciuc, Sebastien Carpentier, Bettina K Wölnerhanssen, Anne Christin Meyer-Gerspach, Ralph Peterli and 2 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Carla E Cadena Del CastilloClinical and Experimental Endocrinology, Department of Chronic Diseases and Metabolism, KU Leuven, Leuven, Belgium.
Onur DenizInstitute of Biotechnology, Helsinki Institute of Life Science, University of Helsinki, Helsinki, Finland.
Femke van GeestClinical and Experimental Endocrinology, Department of Chronic Diseases and Metabolism, KU Leuven, Leuven, Belgium.
Lore RosseelsClinical and Experimental Endocrinology, Department of Chronic Diseases and Metabolism, KU Leuven, Leuven, Belgium.
Ingrid StockmansClinical and Experimental Endocrinology, Department of Chronic Diseases and Metabolism, KU Leuven, Leuven, Belgium.
Marius RobciucInstitute of Biotechnology, Helsinki Institute of Life Science, University of Helsinki, Helsinki, Finland.
Sebastien CarpentierFacility for Systems Biology Based Mass Spectrometry, KU Leuven, Leuven, Belgium.
Bettina K WölnerhanssenSt. Clara Research Ltd, St. Claraspital, Basel, Switzerland.
Anne Christin Meyer-GerspachSt. Clara Research Ltd, St. Claraspital, Basel, Switzerland.
Ralph PeterliClarunis, University Digestive Health Care Center, St. Clara Hospital and University Hospital Basel, Switzerland.
Ville HietakangasInstitute of Biotechnology, Helsinki Institute of Life Science, University of Helsinki, Helsinki, Finland.
Mitsugu ShimobayashiClinical and Experimental Endocrinology, Department of Chronic Diseases and Metabolism, KU Leuven, Leuven, Belgium.

Funding

ChimeraX -- Next Generation Visualization and Analysis Software for Multiscale ModelingR01GM129325 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FERRIN, THOMAS E · 2018 to 2025
$5.2M
NIGMS NIH HHS R01 GM129325
6 · The paper itself

Abstract

The heterodimeric ChREBP-MLX transcription factor complex is a key mediator that couples intracellular sugar levels to carbohydrate and lipid metabolism. To promote the expression of target genes, two ChREBP-MLX heterodimers form a heterotetramer to bind a tandem element with two adjacent E-boxes, called Carbohydrate Responsive Element (ChoRE). How the ChREBP-MLX hetero-tetramerization is achieved and regulated, remains poorly understood. Here we show that MLX phosphorylation on an evolutionarily conserved motif is necessary for the heterotetramer formation on the ChoRE and the transcriptional activity of the ChREBP-MLX complex. We identified CK2 and GSK3 as MLX kinases that coordinately phosphorylate MLX. High intracellular glucose-6-phosphate accumulation inhibits MLX phosphorylation and heterotetramer formation on the ChoRE, impairing ChREBP-MLX activity. Physiologically, MLX phosphorylation is necessary in

Indexed as

Carbohydrate and lipid metabolismChoREChREBPCK2fly developmentMLXsugar tolerance

Identifiers

PMID39282306
PMCPMC11398402

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.