Evidence map›Paper›PMID 39277735›Full record

ArticleBMC nephrology2024

Single-cell RNA sequencing analysis of peripheral blood mononuclear cells in PD-1-induced renal toxicity in patients with lung cancer.

Shusu Liu, Peiyu Lu, Bixia Yang, Yan Yang, Hua Zhou, Min Yang

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Article in BMC nephrology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Shusu Liu *Department of Nephrology, The Third Affiliated Hospital of Soochow University, Changzhou, 213003, China.
Peiyu Lu *Department of Nephrology, The Third Affiliated Hospital of Soochow University, Changzhou, 213003, China.
Bixia YangDepartment of Nephrology, The Third Affiliated Hospital of Soochow University, Changzhou, 213003, China.
Yan YangDepartment of Nephrology, The Third Affiliated Hospital of Soochow University, Changzhou, 213003, China.
Hua ZhouDepartment of Nephrology, The Third Affiliated Hospital of Soochow University, Changzhou, 213003, China.
Min YangDepartment of Nephrology, The Third Affiliated Hospital of Soochow University, Changzhou, 213003, China. yangmin1516@suda.edu.cn.

Funding

the Changzhou Key Medical Discipline No. CZXK202204the Changzhou Science and Technology Program No. CJ20220222the Young Talent Development Plan of Changzhou Health Commission No. CZQM2023002Top Talent of Changzhou "The 14th Five-year Plan" High-Level Health Talents Training Project No. 2022260
6 · The paper itself

Abstract

backgroundAlthough the patient survival rate for many malignancies has been improved with immune checkpoint inhibitors (ICIs), some patients experience various immune-related adverse events (irAEs). IrAEs impact several organ systems, including the kidney. With anti-programmed cell death protein 1 (PD-1) therapy (pembrolizumab), kidney-related adverse events occur relatively rarely compared with other irAEs. However, the occurrence of AKI usually leads to anti-PD-1 therapy interruption or discontinuation. Therefore, there is an urgent need to clarify the mechanisms of renal irAEs (R-irAEs) to facilitate early management. This study aimed to analyse the characteristics of peripheral blood mononuclear cells (PBMCs) in R-irAEs.

methodsPBMCs were collected from three patients who developed R-irAEs after anti-PD-1 therapy and three patients who did not. The PBMCs were subjected to scRNA-seq to identify cell clusters and differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) enrichment analyses were performed to investigate the most active biological processes in immune cells.

resultsFifteen cell clusters were identified across the two groups. FOS, RPS26, and JUN were the top three upregulated genes in CD4

conclusionsIn conclusion, R-irAEs are associated with immune cell dysfunction. DEGs and their enriched pathways identified in CD4

Indexed as

Leukocytes, MononuclearLung NeoplasmsSingle-Cell AnalysisAcute Kidney InjuryAgedAntibodies, Monoclonal, HumanizedFemaleHumansImmune Checkpoint InhibitorsMaleMiddle AgedProgrammed Cell Death 1 ReceptorSequence Analysis, RNAAntibodies, Monoclonal, HumanizedImmune Checkpoint InhibitorsPDCD1 protein, humanpembrolizumabProgrammed Cell Death 1 ReceptorAcute kidney injuryImmune cellsImmune checkpoint inhibitorsImmune-related adverse eventsSingle-cell RNA-seq

Identifiers

PMID39277735
PMCPMC11401319

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.