ArticleNature communications2024
Low-dose cryo-electron ptychography of proteins at sub-nanometer resolution.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- FPGA-based scanner and SerialEM server for 4D-STEM electron tomography.Acta crystallographica. Section D, Structural biology · 2026Article
- CryoEM in the fast lane of structural biology.IUCrJ · 2026Review
- Beyond structure: spectroscopic imaging in cryogenic electron microscopy.Current opinion in structural biology · 2026Review
- Atomic resolution cryo-EM at 200 keV.IUCrJ · 2026Article
- Cryo-EM: the revolution continues.IUCrJ · 2026Review
- Pulsed-electron illumination does not reduce beam damage for imaging biological macromolecules.Nature communications · 2026Article
- CryoWriter: a robotic solution for improved Cryo-EM grid preparation.Nature communications · 2026Article
- Evaluating the Volta phase plate for improved tomogram alignment in cryo-electron tomography.IUCrJ · 2026Article
- Variable Resolution Maps (VRM) in CCTBX and Phenix: Accounting For Local Resolution In cryoEM.bioRxiv : the preprint server for biology · 2026Article
- Microsecond Time-Resolved Cryo-EM Based on Jet Vitrification.bioRxiv : the preprint server for biology · 2025Article
- Development of electron ptychography from algorithms, detectors to its applications.Applied microscopy · 2025Review
- Contrast by electron microscopy in thick biological specimens.Journal of microscopy · 2025Article
- Article
- Electron fourier ptychography for phase reconstruction.Scientific reports · 2025Article
- Dose-efficient cryo-electron microscopy for thick samples using tilt- corrected scanning transmission electron microscopy.Nature methods · 2025Article
- Cryogenic STEM of thick biological specimens.Nature methods · 2025Article
- Phase Imaging Methods in the Scanning Transmission Electron Microscope.Nano letters · 2025Review
- Extending the reach of single-particle cryoEM.Current opinion in structural biology · 2025Review
- Advances in cryo-electron microscopy (cryoEM) for structure-based drug discovery.Expert opinion on drug discovery · 2025Review
- Ptychography at all wavelengths.Nature reviews. Methods primers · 2025Article
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Authors and funding
15 authors.
Funding
Abstract
Cryo-transmission electron microscopy (cryo-EM) of frozen hydrated specimens is an efficient method for the structural analysis of purified biological molecules. However, cryo-EM and cryo-electron tomography are limited by the low signal-to-noise ratio (SNR) of recorded images, making detection of smaller particles challenging. For dose-resilient samples often studied in the physical sciences, electron ptychography - a coherent diffractive imaging technique using 4D scanning transmission electron microscopy (4D-STEM) - has recently demonstrated excellent SNR and resolution down to tens of picometers for thin specimens imaged at room temperature. Here we apply 4D-STEM and ptychographic data analysis to frozen hydrated proteins, reaching sub-nanometer resolution 3D reconstructions. We employ low-dose cryo-EM with an aberration-corrected, convergent electron beam to collect 4D-STEM data for our reconstructions. The high frame rate of the electron detector allows us to record large datasets of electron diffraction patterns with substantial overlaps between the interaction volumes of adjacent scan positions, from which the scattering potentials of the samples are iteratively reconstructed. The reconstructed micrographs show strong SNR enabling the reconstruction of the structure of apoferritin protein at up to 5.8 Å resolution. We also show structural analysis of the Phi92 capsid and sheath, tobacco mosaic virus, and bacteriorhodopsin at slightly lower resolutions.
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