Evidence map›Paper›PMID 39273140›Full record

ArticleInternational journal of molecular sciences2024

Human Beta Defensin-2 mRNA and Proteasome Subunit β Type 8 mRNA Analysis, Useful in Differentiating Skin Biopsies from Atopic Dermatitis and Psoriasis Vulgaris Patients.

Agnieszka Terlikowska-Brzósko, Ryszard Galus, Piotr Murawski, Justyna Niderla-Bielińska, Izabela Młynarczuk-Biały, Elwira Paluchowska, Witold Owczarek

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Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Agnieszka Terlikowska-BrzóskoDepartment of Dermatology, Military Institute of Medicine-National Research Institute, 04-141 Warsaw, Poland.
Ryszard GalusDepartment of Histology and Embryology, Faculty of Medicine, Medical University of Warsaw, 02-004 Warsaw, Poland.
Piotr MurawskiInformation and Communication Technology Department, Military Institute of Medicine-National Research Institute, 04-141 Warsaw, Poland.ORCID 0000-0002-0643-8893
Justyna Niderla-BielińskaDepartment of Histology and Embryology, Faculty of Medicine, Medical University of Warsaw, 02-004 Warsaw, Poland.
Izabela Młynarczuk-BiałyDepartment of Histology and Embryology, Faculty of Medicine, Medical University of Warsaw, 02-004 Warsaw, Poland.ORCID 0000-0002-5192-6415
Elwira PaluchowskaDepartment of Dermatology, Military Institute of Medicine-National Research Institute, 04-141 Warsaw, Poland.
Witold OwczarekDepartment of Dermatology, Military Institute of Medicine-National Research Institute, 04-141 Warsaw, Poland.

Funding

Military Institute of Medicine-National Research Institute 180
6 · The paper itself

Abstract

(1): Atopic dermatitis and psoriasis vulgaris are chronic, inflammatory diseases. Clinical presentation usually leads to a proper diagnosis, but sometimes neither clinical examination nor histopathological evaluation can be conclusive. Therefore, we aimed to build up a novel diagnostic tool and check it for accuracy. The main objective of our work was to differentiate between healthy skin (C), atopic dermatitis (AD) and psoriasis vulgaris (PV) biopsies on the base of involucrin (IVL) and human β-defensin-2 (hBD-2) concentrations and their mRNA, as well as mRNA for TPP2 and PSMB8. (2): ELISA for IVL and hBD-2 proteins and Real-time PCR for the relative expression of mRNA for: IVL (IVL mRNA), hBD-2 (hBD-2 mRNA), PSMB8 (PSMB8 mRNA) and TPP2 (TPP2 mRNA), isolated from skin biopsies taken from AD and PV patients and healthy volunteers were performed. (3): hBD-2 mRNA and PSMB8 mRNA correlated with some parameters of clinical assessment of inflammatory disease severity. hBD-2 mRNA expression, exclusively, was sufficient to distinguish inflammatory skin biopsies from the healthy ones. (4): hBD-2 mRNA and PSMB8 mRNA analysis were the most valuable parameters in differentiating AD and PV biopsies.

Indexed as

beta-DefensinsDermatitis, AtopicPsoriasisRNA, MessengerSkinAdolescentAdultBiopsyDiagnosis, DifferentialFemaleHumansMaleMiddle AgedProteasome Endopeptidase ComplexYoung Adultbeta-DefensinsDEFB4A protein, humanLMP7 proteinProteasome Endopeptidase ComplexRNA, Messengeratopic dermatitis (AD)human beta defensin-2 (hBD-2)involucrin (IVL)proteasome subunit beta type 8 (PSMB8)psoriasis vulgaris (PV)tripeptidyl peptidase 2 (TPP2)

Identifiers

PMID39273140
PMCPMC11395582

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.