Evidence map›Paper›PMID 39273051›Full record

ArticleCells2024

Inhibition of KDM2/7 Promotes Notochordal Differentiation of hiPSCs.

Martha E Diaz-Hernandez, Kimihide Murakami, Shizumasa Murata, Nazir M Khan, Sreekala P V Shenoy, Katrin Henke, Hiroshi Yamada, Hicham Drissi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Martha E Diaz-HernandezDepartment of Orthopaedics, Emory University, Atlanta, GA 30329, USA.ORCID 0000-0003-0225-981X
Kimihide MurakamiDepartment of Orthopaedics, Emory University, Atlanta, GA 30329, USA.
Shizumasa MurataDepartment of Orthopaedics, Emory University, Atlanta, GA 30329, USA.
Nazir M KhanDepartment of Orthopaedics, Emory University, Atlanta, GA 30329, USA.
Sreekala P V ShenoyDepartment of Orthopaedics, Emory University, Atlanta, GA 30329, USA.
Katrin HenkeDepartment of Orthopaedics, Emory University, Atlanta, GA 30329, USA.ORCID 0000-0002-1282-3616
Hiroshi YamadaDepartment of Orthopaedics Surgery, Wakayama Medical University, Wakayama 641-8510, Japan.ORCID 0000-0002-7694-883X
Hicham DrissiDepartment of Orthopaedics, Emory University, Atlanta, GA 30329, USA.

Funding

Emory University NA
6 · The paper itself

Abstract

Intervertebral disc disease (IDD) is a debilitating spine condition that can be caused by intervertebral disc (IVD) damage which progresses towards IVD degeneration and dysfunction. Recently, human pluripotent stem cells (hPSCs) were recognized as a valuable resource for cell-based regenerative medicine in skeletal diseases. Therefore, adult somatic cells reprogrammed into human induced pluripotent stem cells (hiPSCs) represent an attractive cell source for the derivation of notochordal-like cells (NCs) as a first step towards the development of a regenerative therapy for IDD. Utilizing a differentiation method involving treatment with a four-factor cocktail targeting the BMP, FGF, retinoic acid, and Wnt signaling pathways, we differentiate CRISPR/Cas9-generated mCherry-reporter knock-in hiPSCs into notochordal-like cells. Comprehensive analysis of transcriptomic changes throughout the differentiation process identified regulation of histone methylation as a pivotal driver facilitating the differentiation of hiPSCs into notochordal-like cells. We further provide evidence that specific inhibition of histone demethylases KDM2A and KDM7A/B enhanced the lineage commitment of hiPSCs towards notochordal-like cells. Our results suggest that inhibition of KDMs could be leveraged to alter the epigenetic landscape of hiPSCs to control notochord-specific gene expression. Thus, our study highlights the importance of epigenetic regulators in stem cell-based regenerative approaches for the treatment of disc degeneration.

Indexed as

Cell DifferentiationInduced Pluripotent Stem CellsJumonji Domain-Containing Histone DemethylasesNotochordF-Box ProteinsHistone DemethylasesHumansF-Box ProteinsHistone DemethylasesJumonji Domain-Containing Histone DemethylasesKDM2A protein, humanKDM7A protein, humanbulk RNA transcriptomicsdifferentiationepigeneticshuman iPSCintervertebral disc degenerationKDMnotochordal cells

Identifiers

PMID39273051
PMCPMC11393929

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.