Evidence map›Paper›PMID 39272826›Full record

ArticleCancers2024

Impaired DNA Double-Strand Break Repair in Irradiated Sheep Lung Fibroblasts: Late Effects of Previous Irradiation of the Spinal Thecal Sac.

Bassem Youssef, Charbel Feghaly, Joelle Al Choboq, Jolie Bou-Gharios, Rafka Challita, Joyce Azzi, Hanine Bou Hadir, Fabienne Abi Antoun, Tarek Araji, Phillip J Taddei and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancers, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Bassem YoussefDepartment of Radiation Oncology, American University of Beirut Medical Center, Beirut 1107 2020, Lebanon.ORCID 0000-0003-3960-8155
Charbel FeghalyDepartment of Radiation Oncology, American University of Beirut Medical Center, Beirut 1107 2020, Lebanon.ORCID 0000-0003-2789-1512
Joelle Al ChoboqDepartment of Radiation Oncology, American University of Beirut Medical Center, Beirut 1107 2020, Lebanon.ORCID 0000-0001-6772-6947
Jolie Bou-GhariosDepartment of Radiation Oncology, American University of Beirut Medical Center, Beirut 1107 2020, Lebanon.ORCID 0000-0002-9984-913X
Rafka ChallitaDepartment of Radiation Oncology, American University of Beirut Medical Center, Beirut 1107 2020, Lebanon.ORCID 0000-0002-2918-5964
Joyce AzziDepartment of Radiation Oncology, American University of Beirut Medical Center, Beirut 1107 2020, Lebanon.
Hanine Bou HadirDepartment of Radiation Oncology, American University of Beirut Medical Center, Beirut 1107 2020, Lebanon.ORCID 0009-0000-9001-3865
Fabienne Abi AntounDepartment of Radiation Oncology, American University of Beirut Medical Center, Beirut 1107 2020, Lebanon.ORCID 0009-0008-2403-1318
Tarek ArajiDepartment of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon.ORCID 0000-0003-3339-2680
Phillip J TaddeiDepartment of Radiation Oncology, American University of Beirut Medical Center, Beirut 1107 2020, Lebanon.
Fady GearaDepartment of Radiation Oncology, American University of Beirut Medical Center, Beirut 1107 2020, Lebanon.
Pierre SfeirDepartment of Surgery, American University of Beirut Medical Center, Beirut 1107 2020, Lebanon.ORCID 0000-0001-7989-0322
Abdo JurjusDepartment of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon.
Wassim Abou-KheirDepartment of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon.ORCID 0000-0001-9719-9324
Larry BodgiDepartment of Radiation Oncology, American University of Beirut Medical Center, Beirut 1107 2020, Lebanon.ORCID 0000-0003-1067-9435

Funding

Radiotherapy outcomes for children in developing versus developed countriesK01TW008409 · FIC · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI TADDEI, PHILLIP J · 2010 to 2013
$447k
American University of Beirut 103949FIC NIH HHS 1K01TW008409lebanese atomic energy commission NALebanese national counsil for scientific research 2505Nayef K Basile Foundation NA
6 · The paper itself

Abstract

Children with cancer previously treated with radiotherapy face the likelihood of side effects that can be debilitating or fatal. This study aimed to assess the long-term effect of medulloblastoma radiotherapy on the DNA double-strand break (DSB) repair capability of primary fibroblasts derived from lung biopsies of previously irradiated young sheep. This study included biopsies from three control and five irradiated sheep. The treated sheep had previously received spinal radiotherapy at a total dose of 28 Gy, which is equivalent to pediatric medulloblastoma treatment. Lung biopsies were taken 4 years post-irradiation from high-dose (HD, >18 Gy) and low-dose (LD, <2 Gy) regions. Fifteen cell lines were extracted (six control, four LD and five HD). The cells were irradiated, and DNA DSB repair was analyzed by immunofluorescence. Clonogenic, trypan blue and micronuclei assays were performed. Both the HD and LD cell lines had a significantly higher number of residual γH2AX foci 24 h and a significant decrease in pATM activity post-irradiation compared to the control. There was no statistically significant difference in the clonogenic assay, trypan blue and micronuclei results. Our study showed that a previous irradiation can impair the DNA DSB repair mechanism of ovine lung fibroblasts.

Indexed as

DNA double-strand breaksDNA repairpediatric cancerradiosensitivityradiotherapy

Identifiers

PMID39272826
PMCPMC11394103

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.