ArticleNature chemical biology2025
Incorporation of nitrogen in antinutritional Solanum alkaloid biosynthesis.
Article in Nature chemical biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Biosynthetic mechanism and ecological function of chirality in Solanum steroidal alkaloids.Nature communications · 2026Article
- The Role of Two Glycoalkaloid Metabolism Genes in α-Tomatine Biosynthesis and Basal Defence in Tomato.Molecular plant pathology · 2026Article
- Rewiring Steroidal Metabolic Pathways for Diosgenin Production in Solanum nigrum.Plant biotechnology journal · 2026Article
- Structural innovation and flexibility in plant chemical defenses.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Structural innovation in the evolution of plant chemical defense.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Glycoalkaloid-Free Starch Potatoes Generated by CRISPR/Cas9-Mediated Mutations of Genes in the Glycoalkaloid Biosynthesis Pathway Enable More Sustainable Uses of By-Products From Starch Production.Plant biotechnology journal · 2026Article
- Complex engineering of Solanum alkaloids structural diversity in Nicotiana benthamiana.Plant biotechnology journal · 2026Article
- Decoding steroid-derived metabolite engineering inHorticulture research · 2025Article
- A million shades of green: understanding and harnessing plant metabolic diversity.The EMBO journal · 2025Review
- Identification of genes involved in verazine biosynthesis in Veratrum grandiflorum and their heterologous production in Saccharomyces cerevisiae.BMC plant biology · 2025Article
- Physiological and Molecular Mechanisms of Light-Induced Greening in Potatoes: A Path to Food Safety.Foods (Basel, Switzerland) · 2025Review
- Synthetic Biology in Natural Product Biosynthesis.Chemical reviews · 2025Review
- Alkaloid production of Solanum elaeagnifolium Cav from callus for anticancer potential using gene expression of cancer-related genes.PloS one · 2025Article
- Potato steroidal glycoalkaloids: properties, biosynthesis, regulation and genetic manipulation.Molecular horticulture · 2024Review
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16 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Steroidal glycoalkaloids (SGAs) are specialized metabolites produced by hundreds of Solanum species including food crops, such as tomato, potato and eggplant. Unlike true alkaloids, nitrogen is introduced at a late stage of SGA biosynthesis through an unknown transamination reaction. Here, we reveal the mechanism by which GLYCOALKALOID METABOLISM12 (GAME12) directs the biosynthesis of nitrogen-containing steroidal alkaloid aglycone in Solanum. We report that GAME12, a neofunctionalized γ-aminobutyric acid (GABA) transaminase, undergoes changes in both active site specificity and subcellular localization to switch from its renown and generic activity in core metabolism to function in a specialized metabolic pathway. Moreover, overexpression of GAME12 alone in engineered S. nigrum leaves is sufficient for de novo production of nitrogen-containing SGAs. Our results highlight how hijacking a core metabolism GABA shunt enzyme is crucial in numerous Solanum species for incorporating a nitrogen to a steroidal-specialized metabolite backbone and form defensive alkaloids.
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