Evidence map›Paper›PMID 39271811›Full record

ArticleCommunications biology2024

PGC-based cryobanking, regeneration through germline chimera mating, and CRISPR/Cas9-mediated TYRP1 modification in indigenous Chinese chickens.

Keiji Kinoshita, Kumiko Tanabe, Yoshiaki Nakamura, Ken-Ichi Nishijima, Takayuki Suzuki, Yuya Okuzaki, Shusei Mizushima, Ming-Shan Wang, Sami Ullah Khan, Kaixiang Xu and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Article
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  5. Review
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  7. Manipulating primordial germ cells from chicken and other bird species.The Journal of reproduction and development · 2026
    Review
  8. Review
  9. Review
  10. Article
  11. Article
  12. Review
  13. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Keiji Kinoshita *State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.
Kumiko Tanabe *State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.
Yoshiaki NakamuraLaboratory of Animal Breeding and Genetics, Graduate School of Integrated Sciences for Life and School of Applied Biological Science, Hiroshima University, Hiroshima, 739-8528, Japan.
Ken-Ichi NishijimaAvian Bioscience Research Center, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.
Takayuki SuzukiDepartment of Biology, Graduate School of Science, Osaka Metropolitan University, Osaka, 558-8585, Japan.
Yuya OkuzakiAvian Bioscience Research Center, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.
Shusei MizushimaDepartment of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo, 060-0810, Japan.
Ming-Shan WangState Key Laboratory of Genetic resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China.
Sami Ullah KhanState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.
Kaixiang XuState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.
Muhammad Ameen JamalState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.
Taiyun WeiState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.
Heng ZhaoState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.
Yanhua SuState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.
Feizhou SunNational Center for Preservation of Animal Genetic Resources, National Animal Husbandry Service, Beijing, 100125, China.
Gang LiuNational Center for Preservation of Animal Genetic Resources, National Animal Husbandry Service, Beijing, 100125, China.
Fangxian ZhuNational Center for Preservation of Animal Genetic Resources, National Animal Husbandry Service, Beijing, 100125, China.
Hong-Ye ZhaoState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, 650201, China. hyzhao2000@126.com.ORCID 0000-0002-5900-3033
Hong-Jiang WeiState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan, 650201, China. hongjiangwei@126.com.ORCID 0000-0002-5663-1093

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Primordial germ cells (PGCs) are vital for producing sperm and eggs and are crucial for conserving chicken germplasm and creating genetically modified chickens. However, efforts to use PGCs for preserving native chicken germplasm and genetic modification via CRISPR/Cas9 are limited. Here we show that we established 289 PGC lines from eight Chinese chicken populations with an 81.6% success rate. We regenerated Piao chickens by repropagating cryopreserved PGCs and transplanting them into recipient chickens, achieving a 12.7% efficiency rate. These regenerated chickens carried mitochondrial DNA from female donor PGC and the rumplessness mutation from both male and female donors. Additionally, we created the TYRP1 (tyrosinase-related protein 1) knockout (KO) PGC lines via CRISPR/Cas9. Transplanting KO cells into male recipients and mating them with wild-type hens produced four TYRP1 KO chickens with brown plumage due to reduced eumelanin production. Our work demonstrates efficient PGC culture, cryopreservation, regeneration, and gene editing in chickens.

Indexed as

ChickensCRISPR-Cas SystemsCryopreservationGerm CellsAnimalsAnimals, Genetically ModifiedChimeraFemaleGene EditingGene Knockout TechniquesMaleOxidoreductasesRegenerationOxidoreductasestyrosinase-related protein-1

Identifiers

PMID39271811
PMCPMC11399235

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.