Evidence map›Paper›PMID 39271727›Full record

ArticleNature communications2024

Interferometric excitation fluorescence lifetime imaging microscopy.

Pavel Malý, Dita Strachotová, Aleš Holoubek, Petr Heřman

Abstract read
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Hyperspectral Fluorescence Imaging with a New Polarity-Ultrasensitive Fluorescent Probe.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Pavel MalýFaculty of Mathematics and Physics, Institute of Physics, Charles University, Prague, Czech Republic. pavel.maly@mff.cuni.cz.ORCID http://orcid.org/0000-0001-9244-9718
Dita StrachotováFaculty of Mathematics and Physics, Institute of Physics, Charles University, Prague, Czech Republic.
Aleš HoloubekDepartment of Proteomics, Institute of Hematology and Blood Transfusion, Prague, Czech Republic.ORCID http://orcid.org/0000-0003-0642-9062
Petr HeřmanFaculty of Mathematics and Physics, Institute of Physics, Charles University, Prague, Czech Republic.

Funding

EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020) 101030656Grantová Agentura České Republiky (Grant Agency of the Czech Republic) 22-03875S
6 · The paper itself

Abstract

Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique with numerous imaging applications. Yet, one of the limitations of FLIM is that it only provides information about the emitting state. Here, we present an extension of FLIM by interferometric measurement of fluorescence excitation spectra. Interferometric Excitation Fluorescence Lifetime Imaging Microscopy (ixFLIM) reports on the correlation of the excitation spectra and emission lifetime, providing the correlation between the ground-state absorption and excited-state emission. As such, it extends the applicability of FLIM and removes some of its limitations. We introduce ixFLIM on progressively more complex systems, directly compare it to standard FLIM, and apply it to quantitative resonance energy transfer imaging from a single measurement.

Identifiers

PMID39271727
PMCPMC11399241

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.