Nanozymes: Classification and Analytical Applications - A Review.

The recent discovery of a new class of nanomaterials called nanozymes, which have the action of enzymes and are thus of tremendous significance, has altered our understanding of these previously believed to be biologically inert nanomaterials. As a significant and exciting class of synthetic enzymes, nanozymes have distinct advantages over natural enzymes. They are less expensive, more stable, and easier to work with and store, making them a viable substitute. This practical advantage of nanozymes over natural enzymes reassures us about the potential of this new technology. Peroxidase-like nanozymes have been investigated for the purpose of creating adaptable biosensors via the use of molecularly imprinted polymers (MIPs) or particular bio recognition ligands, including enzymes, antibodies, and aptamers. This review delves into the distinctions between synthetic and natural enzymes, explaining their structures and analytical applications. It primarily focuses on carbon-based nanozymes, particularly those that contain both carbon and hydrogen, as well as metal-based nanozymes like Fe, Cu, and Au, along with their metal oxide (FeO, CuO), which have applications in many fields today. Analytical chemistry finds great use for nanozymes for sensing and other applications, particularly in comparison with other classical methods in terms of selectivity and sensitivity. Nanozymes, with their unique catalytic capabilities, have emerged as a crucial tool in the early diagnosis of COVID-19. Their application in nanozyme-based sensing and detection, particularly through colorimetric and fluorometric methods, has significantly advanced our ability to detect the virus at an early stage.