ArticleRice (New York, N.Y.)2024
OsWRKY70 Plays Opposite Roles in Blast Resistance and Cold Stress Tolerance in Rice.
Article in Rice (New York, N.Y.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Harnessing CRISPR-Cas technology to enhance rice resilience under abiotic and biotic stress.Journal of applied genetics · 2026Review
- Advances in Genome Editing for Plant Disease Resistance Breeding.Plants (Basel, Switzerland) · 2026Review
- The GhWRKY70-International journal of molecular sciences · 2026Article
- Cloning ofGenes · 2026Article
- Impairment of the Uroporphyrinogen Decarboxylase 2 Gene Induces Lesion Mimicry and Downregulates Tetrapyrrole Biosynthesis Pathway Genes in Rice.Rice (New York, N.Y.) · 2026Article
- Advances in CRISPR/Cas systems for engineering abiotic stress tolerance in plants: mechanisms and future prospects.Planta · 2026Review
- Transcriptome Analysis IdentifiesPlants (Basel, Switzerland) · 2026Article
- Review
- Protein Post-Translational Modifications in Plant Abiotic Stress Responses.Plants (Basel, Switzerland) · 2025Review
- Effect of developmental dynamics on WRKY expression in barley with varying phenologies and trichome micromorphologies.BMC plant biology · 2025Article
- Functional Characterization of OsWRKY7, a Novel WRKY Transcription Factor in Rice.Life (Basel, Switzerland) · 2025Article
- A loss-of-function of ZmWRKY125 induced by CRISPR/Cas9 improves resistance against Fusarium verticillioides in maize kernels.Plant cell reports · 2025Article
- The Role of Genetic Resistance in Rice Disease Management.International journal of molecular sciences · 2025Review
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7 authors.
Funding
Abstract
The transcription factor WRKYs play pivotal roles in the adapting to adverse environments in plants. Prior research has demonstrated the involvement of OsWRKY70 in resistance against herbivores and its response to abiotic stress. Here, we reported the functional analysis of OsWRKY70 in immunity against fungal diseases and cold tolerance. The results revealed that OsWRKY70 was induced by various Magnaporthe oryzae strains. Knock out mutants of OsWRKY70, which were generated by the CRISPR/Cas9 system, exhibited enhanced resistance to M. oryzae. This was consistent with fortifying the reactive oxygen species (ROS) burst after inoculation in the mutants, elevated transcript levels of defense-responsive genes (OsPR1b, OsPBZ1, OsPOX8.1 and OsPOX22.3) and the observation of the sluggish growth of invasive hyphae under fluorescence microscope. RNA sequencing (RNA-seq) and quantitative real-time PCR (qRT-PCR) validations demonstrated that differentially expressed genes were related to plant-pathogen interactions, hormone transduction and MAPK cascades. Notably, OsbHLH6, a key component of the JA signaling pathway, was down-regulated in the mutants compared to wild type plants. Further investigation confirmed that OsWRKY70 bound to the promoter of OsbHLH6 by semi-in vivo chromatin immunoprecipitation (ChIP). Additionally, the loss-function of OsWRKY70 impaired cold tolerance in rice. The enhanced susceptibility in the mutants characterized by excessive ROS production, elevated ion leakage rate and increased malondialdehyde content, as well as decreased activity of catalase (CAT) and peroxidase (POD) under low temperature stress was, which might be attributed to down-regulation of cold-responsive genes (OsLti6b and OsICE1). In conclusion, our findings indicate that OsWRKY70 negatively contributes to blast resistance but positively regulates cold tolerance in rice, providing a strategy for crop breeding with tolerance to stress.
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