ArticleThe Journal of cell biology1985
Components of the cytoskeleton in the retinal pigmented epithelium of the chick.
Article in The Journal of cell biology, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- A novel terminal web-like structure in cortical lens fibers: architecture and functional assessment.Anatomical record (Hoboken, N.J. : 2007) · 2010Article
- Involvement of the mutated M protein in altered budding polarity of a pantropic mutant, F1-R, of Sendai virus.Journal of virology · 1996Article
- Possible involvement of microtubule disruption in bipolar budding of a Sendai virus mutant, F1-R, in epithelial MDCK cells.Journal of virology · 1993Article
- Molecular heterogeneity of the actin filament cytoskeleton associated with microvilli of photoreceptors, Müller's glial cells and pigment epithelial cells of the retina.Histochemistry · 1993Article
- Apical polarity of Na,K-ATPase in retinal pigment epithelium is linked to a reversal of the ankyrin-fodrin submembrane cytoskeleton.The Journal of cell biology · 1991Article
- Ultrastructural and immunocytochemical analysis of the circumferential microfilament bundle in avian retinal pigmented epithelial cells in vitro.Cell and tissue research · 1991Article
- Isolation of a 5-kilodalton actin-sequestering peptide from human blood platelets.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Morphological changes in the zonula adhaerens during embryonic development of chick retinal pigment epithelial cells.Cell and tissue research · 1990Article
- Cytoskeletons of retinal pigment epithelial cells: interspecies differences of expression patterns indicate independence of cell function from the specific complement of cytoskeletal proteins.Cell and tissue research · 1988Article
- The cytoskeleton of chick retinal pigment epithelial cells in situ.Cell and tissue research · 1987Article
- Microtubules and actin filaments are not critically involved in the biogenesis of epithelial cell surface polarity.The Journal of cell biology · 1986Article
- Changes in membrane-microfilament interaction in intercellular adherens junctions upon removal of extracellular Ca2+ ions.The Journal of cell biology · 1986Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The retinal pigmented epithelium (RPE) is a simple cuboidal epithelium with apical processes which, unlike many epithelia, do not extend freely into a lumen but rather interdigitate closely with the outer segments of the neural retina. To determine whether this close association was reflected in the cytoskeletal organization of the RPE, we studied the components of the cytoskeleton of the RPE and their localization in the body of the cell and in the apical processes. By relative mobility on SDS gels and by immunoblotting, we identified actin, vimentin, myosin, spectrin (240/235), and alpha-actinin as major components, and vinculin as a minor component. In addition, the RPE cytoskeleton contains polypeptides of Mr 280,000 and 250,000; the latter co-electrophoreses with actin-binding protein. By immunofluorescence, the terminal web region appeared similar to the comparable region of the intestinal epithelium that consists of broad belts of microfilaments containing myosin, actin, spectrin, and alpha-actinin. However, the components of the apical processes were very different from those of intestinal microvilli. We observed staining along the process for myosin, actin, spectrin, alpha-actinin, and vinculin. The presence in the apical processes of contractile proteins and also of proteins typically found at sites of cell attachments suggests that the RPE may actively adhere to, and exert tension on, the neural retina.
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