Observational studyItalian journal of pediatrics2024
Comparison of targeted next-generation sequencing and metagenomic next-generation sequencing in the identification of pathogens in pneumonia after congenital heart surgery: a comparative diagnostic accuracy study.
Observational study in Italian journal of pediatrics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Metagenomic Next-Generation Sequencing Versus Conventional Microbiological Tests for Pathogen Identification and Prognostic Evaluation in Pediatric Patients with Post-Cardiac Surgery Infections: A Retrospective Cohort Study.Journal of clinical medicine · 2026Article
- Pneumonia with BALF Detection ofInfection and drug resistance · 2026Article
- Application value of next generation sequencing technology for pathogen detection in patients with pulmonary infection and lung cancer.American journal of cancer research · 2026Article
- Next-generation sequencing in the etiological diagnosis of severe childhood pneumonia.Frontiers in pediatrics · 2026Review
- tNGS-based detection of respiratory pathogens in a single center: associations with age, gender, season, and co-infections.Frontiers in cellular and infection microbiology · 2025Article
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Authors and funding
4 authors.
Funding
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Abstract
backgroundThis study aimed to compare targeted next-generation sequencing (tNGS) with metagenomic next-generation sequencing (mNGS) for pathogen detection in infants with severe postoperative pneumonia after congenital heart surgery.
methodsWe conducted a retrospective observational study using data from the electronic medical record system of infants who developed severe pneumonia after surgery for congenital heart disease from August 2021 to August 2022. Infants were divided into tNGS and mNGS groups based on the pathogen detection methods. The primary outcome was the efficiency of pathogen detection, and the secondary outcomes were the timeliness and cost of each method.
resultsIn the study, 91 infants were included, with tNGS detecting pathogens in 84.6% (77/91) and mNGS in 81.3% (74/91) of cases (P = 0.55). No significant differences were found in sensitivity, specificity, PPA, and NPA between the two methods (P > 0.05). tNGS identified five strains with resistance genes, while mNGS detected one strain. Furthermore, tNGS had a faster detection time (12 vs. 24 h) and lower cost ($150 vs. $500) compared to mNGS.
conclusiontNGS offers similar sensitivity to mNGS but with greater efficiency and cost-effectiveness, making it a promising approach for respiratory pathogen detection.
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