Evidence map›Paper›PMID 39266872›Full record

ArticleCell biochemistry and biophysics2024

In vitro Chondrogenic Induction Promotes the Expression Level of IL-10 via the TGF-β/SMAD and Canonical Wnt/β-catenin Signaling Pathways in Exosomes Secreted by Human Adipose Tissue-derived Mesenchymal Stem Cells.

Tugba Semerci Sevimli, Ulukan Inan, Dilara Mantar, Kubra Guler, Zarifa Ahmadova, Kadri Gulec, Ahmet Emin Topal

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Article in Cell biochemistry and biophysics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Tugba Semerci SevimliCellular Therapy and Stem Cell Production Application and Research Center (ESTEM), Eskisehir Osmangazi University, 26040, Eskisehir, Turkey. drtugbasevimli@gmail.com.ORCID http://orcid.org/0000-0003-4856-2304
Ulukan InanDepartment of Orthopedics and Traumatology, Faculty of Medicine, Eskisehir Osmangazi University, 26040, Eskisehir, Turkey.ORCID http://orcid.org/0000-0002-1903-5516
Dilara MantarErzurum Technical University, Erzurum, Turkey.
Kubra GulerDepartment of Biochemistry, School of Pharmacy, Bahcesehir University, Istanbul, Turkey.
Zarifa AhmadovaDepartment of Surgery, Medical Faculty Mannheim, Heidelberg University, 68167, Mannheim, Germany.ORCID http://orcid.org/0000-0003-0883-2752
Kadri GulecDepartment of Analytical Chemistry, Faculty of Pharmacy, Anadolu University, 26470, Eskisehir, Turkey.ORCID http://orcid.org/0000-0002-1392-8276
Ahmet Emin TopalDepartment of Biochemistry, School of Pharmacy, Bahcesehir University, Istanbul, Turkey.ORCID http://orcid.org/0000-0001-9951-0171

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Current treatment approaches cannot exactly regenerate cartilage tissue. Regarding some problems encountered with cell therapy, exosomes are advantageous because of their "cell-free" nature. This study examines the relationship between IL-10 and TGF-β and Canonical Wnt/β-catenin signal pathways in human adipose tissue-derived MSCs exosomes (hAT-MSCs-Exos) after in vitro chondrogenic differentiation. Human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and, as a control group, human fetal chondroblast cells (hfCCs) were differentiated chondrogenically in vitro. Exosome isolation and characterization analyses were performed. Chondrogenic differentiation was shown by Alcian Blue and Safranin O stainings. The expression levels of IL-10, TGF-β/SMAD signaling pathway genes, and Canonical Wnt/β-catenin signaling pathway genes, which play an essential role in chondrogenesis, were analyzed by RT-qPCR. Conditioned media cytokine levels were measured by using the TGF-β and IL-10 ELISA kits. IL-10 expression was upregulated in both chondrogenic differentiated hAT-MSC-Exos (dhAT-MSC-Exos) (p < 0.0001). In the TGF-β signaling pathway, TGF-β (p < 0.0001), SMAD2 (p < 0.0001), SMAD4 (p < 0.001), ACAN (p < 0.0001), SOX9 (p < 0.05) and COL1A2 (p < 0.0001) expressions were upregulated in dhAT-MSC-Exos. SMAD3 expression was upregulated in non-differentiated hAT-MSC-Exos. In the Canonical Wnt/β-catenin signaling pathway, WNT (p < 0.0001) and CTNNB1(p < 0.0001) expressions were upregulated in dhAT-MSC-Exos. AXIN (p < 0.0001) expression was upregulated in non-differentiated hAT-MSC-Exos. TGF-β and IL-10 levels were higher in dhAT-MSCs) (p < 0.0001). Related to these results, IL-10 may induce TGF-β/SMAD and Canonical Wnt/β-catenin signaling pathways in hAT-MSC exosomes obtained after chondrogenic differentiation. Therefore, using these exosomes for cartilage regeneration can lead to the development of treatment methods.

Indexed as

Adipose TissueCell DifferentiationChondrogenesisExosomesInterleukin-10Mesenchymal Stem CellsTransforming Growth Factor betaWnt Signaling Pathwaybeta CateninCells, CulturedHumansSmad3 ProteinSmad ProteinsSOX9 Transcription Factorbeta CateninIL10 protein, humanInterleukin-10Smad3 ProteinSmad ProteinsSOX9 Transcription FactorTransforming Growth Factor betaCanonical Wnt/β-catenin signalingChondrogenesisIL-10Mesenchymal stem cell-exosomeTGF-β signaling pathway

Identifiers

PMID39266872

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.