Evidence map›Paper›PMID 39261879›Full record

ArticleActa veterinaria Scandinavica2024

Enhancing canine semen quality through a second centrifugation after 48 hours of storage: a comparative study.

Letizia Sinagra, Angela Polisca, Giulia Donato, Tiziana Caspanello, Giorgia Pettina, Sara Pastore, Massimo De Majo, Santo Cristarella, Marco Quartuccio, Viola Zappone

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta veterinaria Scandinavica, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Letizia SinagraDepartment of Veterinary Sciences, University of Messina, Viale Palatucci, 13, Messina, 98168, Italy.
Angela PoliscaDepartment of Veterinary Medicine, University of Perugia, Via San Costanzo 4, Perugia, 06126, Italy.
Giulia DonatoDepartment of Veterinary Sciences, University of Messina, Viale Palatucci, 13, Messina, 98168, Italy.
Tiziana CaspanelloDepartment of Veterinary Sciences, University of Messina, Viale Palatucci, 13, Messina, 98168, Italy. tiziana.caspanello@studenti.unime.it.ORCID http://orcid.org/0000-0003-1801-4454
Giorgia PettinaDepartment of Veterinary Sciences, University of Messina, Viale Palatucci, 13, Messina, 98168, Italy.
Sara PastoreDepartment of Veterinary Medicine, University of Perugia, Via San Costanzo 4, Perugia, 06126, Italy.
Massimo De MajoDepartment of Veterinary Sciences, University of Messina, Viale Palatucci, 13, Messina, 98168, Italy.
Santo CristarellaDepartment of Veterinary Sciences, University of Messina, Viale Palatucci, 13, Messina, 98168, Italy.
Marco QuartuccioDepartment of Veterinary Sciences, University of Messina, Viale Palatucci, 13, Messina, 98168, Italy.
Viola ZapponeDepartment of Veterinary Sciences, University of Messina, Viale Palatucci, 13, Messina, 98168, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCentrifugation is a common procedure to improve the quality of chilled and frozen canine semen by removing debris and seminal plasma and adding semen extenders. The aim of this study was to evaluate the efficacy and influence of a second centrifugation after 48 h of storage at 5 °C on the sperm quality of canine semen. The ejaculates of 45 healthy male dogs, divided into three groups according to body weight, were analyzed for macro- and microparameters such as ejaculate volume, sperm concentration, kinematic parameters, morphology, and integrity of plasma membrane. Samples were analyzed at baseline conditions (T

resultsThe results showed a significant effect of a second centrifugation on the improvement of chilled sperm quality compared to the other techniques, especially up to 48 h.

conclusionsAnalysis of the data showed that the semen samples centrifuged and then cooled at 5 °C had acceptable semen parameters, especially in terms of motility, with a gradual decrease in serial evaluations after 24 and 48 h. A second centrifugation after 48 h of storage may lead to better semen quality and improve the kinetics of sperm parameters, the percentage of morphologically normal sperm and the percentage of sperm with intact membranes.

Indexed as

CentrifugationSemen AnalysisSemen PreservationAnimalsDogsMaleSemenSpermatozoaSperm MotilityTime FactorsArtificial inseminationCooled spermDogSperm improvingSperm motility

Identifiers

PMID39261879
PMCPMC11389515

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.