Evidence map›Paper›PMID 39261761›Full record

ArticleBMC cancer2024

TOB1 inhibits the gastric cancer progression by focal adhesion pathway and ERK pathway based on transcriptional and metabolic sequencing.

Hongjie He, Kexian Dong, Mingming Chen, Yuanyuan Wang, Yawen Li, Dong Wang, Mansha Jia, Xiangning Meng, Wenjing Sun, Songbin Fu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Hongjie He *Scientific Research Centre, the Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, China.
Kexian Dong *Key Laboratory of Preservation of Human Genetic Resources and Disease Control in China, Ministry of Education, Harbin Medical University, Harbin, 150081, China.
Mingming ChenScientific Research Centre, the Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, China.
Yuanyuan WangScientific Research Centre, the Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, China.
Yawen LiScientific Research Centre, the Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, China.
Dong WangScientific Research Centre, the Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, China.
Mansha JiaScientific Research Centre, the Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, China.
Xiangning MengKey Laboratory of Preservation of Human Genetic Resources and Disease Control in China, Ministry of Education, Harbin Medical University, Harbin, 150081, China.
Wenjing SunKey Laboratory of Preservation of Human Genetic Resources and Disease Control in China, Ministry of Education, Harbin Medical University, Harbin, 150081, China.
Songbin FuKey Laboratory of Preservation of Human Genetic Resources and Disease Control in China, Ministry of Education, Harbin Medical University, Harbin, 150081, China.
Jingcui YuScientific Research Centre, the Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, China. yujingcui@ems.hrbmu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gastric cancer is one of the most malignant digestive tract tumors worldwide and its progression is associated with gene expression and metabolic alteration. We revealed that the gastric cancer patients with lower expression level of TOB1 exhibited poorer overall survivals according to the data in Kaplan-Meier Plotter. The unphosphorylated TOB1 protein which is effective expressed lower in gastric cancer cells. The gastric cancer cells with TOB1 gene depletion performed higher abilities of proliferation, migration and invasion and lower ability of apoptosis in vitro. The TOB1 gene depletion also promoted the tumorigenesis of gastric cancer cells in vivo. The gastric cancer cells with TOB1 gene overexpression had the converse behaviors. The transcriptional and metabolic sequencing was performed. The analyzation results showed that genes correlate-expressed with TOB1 gene were enriched in the pathways related to ERK pathway, including focal adhesion pathway, which was verified using real-time quantitative PCR. After inhibiting ERK pathway, the proliferation, colony formation and migration abilities were reduced in gastric cancer cells with low phosphorylated TOB1 protein expression level. Moreover, Pearson correlation analysis was adopted to further analyze the correlation of enriched metabolic products and differentially expressed genes. The expression of Choline, UDP-N-acetylglucosamine, Adenosine and GMP were related to the function of TOB1. This study demonstrates the genes and metabolites related to focal adhesion pathway and ERK pathway are the potential diagnosis and therapeutic targets to gastric cancer with TOB1 depletion.

Indexed as

Cell MovementCell ProliferationDisease ProgressionGene Expression Regulation, NeoplasticMAP Kinase Signaling SystemStomach NeoplasmsTumor Suppressor ProteinsAnimalsApoptosisCell Line, TumorFocal AdhesionsHumansIntracellular Signaling Peptides and ProteinsMiceIntracellular Signaling Peptides and ProteinsTOB1 protein, humanTumor Suppressor ProteinsERK pathwayFocal adhesion pathwayGastric cancerMetabolitesTOB1

Identifiers

PMID39261761
PMCPMC11389266

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.