Evidence map›Paper›PMID 39261738›Full record

ArticleNature2024

Multi-pass, single-molecule nanopore reading of long protein strands.

Keisuke Motone, Daphne Kontogiorgos-Heintz, Jasmine Wee, Kyoko Kurihara, Sangbeom Yang, Gwendolin Roote, Oren E Fox, Yishu Fang, Melissa Queen, Mattias Tolhurst and 3 more

Abstract read
In one paragraph

Article in Nature, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.

0numbers the graph read from it
0cells of the map it votes in
75citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

75 citing papers in PubMed.

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15 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Keisuke Motone *Paul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.ORCID 0000-0002-8277-0599
Daphne Kontogiorgos-Heintz *Paul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.ORCID 0009-0006-6874-7575
Jasmine WeePaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.
Kyoko KuriharaPaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.ORCID 0009-0001-3838-1967
Sangbeom YangPaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.
Gwendolin RootePaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.
Oren E FoxPaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.ORCID 0000-0002-1558-7962
Yishu FangPaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.
Melissa QueenPaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.
Mattias TolhurstMolecular Engineering and Science Institute, University of Washington, Seattle, WA, USA.
Nicolas CardozoMolecular Engineering and Science Institute, University of Washington, Seattle, WA, USA.
Miten JainDepartment of Bioengineering, Department of Physics, Northeastern University, Boston, MA, USA.ORCID 0000-0002-4571-3982
Jeff NivalaPaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA. jmdn@cs.washington.edu.ORCID 0000-0002-8210-5417

Funding

Long-read single-molecule protein sequencing on an array of unfoldase-coupled nanoporesR01HG012545 · NHGRI · UNIVERSITY OF WASHINGTON · PI NIVALA, JEFFREY MATTHEW · 2022 to 2024
$1.8M
National Science FoundationNHGRI NIH HHS R01 HG012545
6 · The paper itself

Abstract

The ability to sequence single protein molecules in their native, full-length form would enable a more comprehensive understanding of proteomic diversity. Current technologies, however, are limited in achieving this goal

Indexed as

NanoporesProteinsSequence Analysis, ProteinSingle Molecule ImagingAmino Acid SubstitutionATPases Associated with Diverse Cellular ActivitiesEndopeptidase ClpEscherichia coli ProteinsLipoproteinsMolecular ChaperonesPhosphorylationProtein DomainsProtein Processing, Post-TranslationalATPases Associated with Diverse Cellular ActivitiesClpX protein, E coliCsgG protein, E coliEndopeptidase ClpEscherichia coli ProteinsLipoproteinsMolecular ChaperonesProteins

Identifiers

PMID39261738
PMCPMC11410661

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.