Evidence map›Paper›PMID 39261551›Full record

ArticleScientific reports2024

Development and validation of an experimental life support system to study coral reef microbial communities.

T M Stuij, D F R Cleary, R J M Rocha, A R M Polonia, D A Machado E Silva, J C Frommlet, A Louvado, Y M Huang, N J De Voogd, N C M Gomes

Abstract readValidation Study
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

T M StuijCentre for Environmental and Marine Studies (CESAM) and Department of Biology, University of Aveiro, Campus Universitário Santiago, 3810-193, Aveiro, Portugal. tamarastuij@ua.pt.
D F R ClearyCentre for Environmental and Marine Studies (CESAM) and Department of Biology, University of Aveiro, Campus Universitário Santiago, 3810-193, Aveiro, Portugal.
R J M RochaCentre for Environmental and Marine Studies (CESAM) and Department of Biology, University of Aveiro, Campus Universitário Santiago, 3810-193, Aveiro, Portugal.
A R M PoloniaCentre for Environmental and Marine Studies (CESAM) and Department of Biology, University of Aveiro, Campus Universitário Santiago, 3810-193, Aveiro, Portugal.
D A Machado E SilvaCentre for Environmental and Marine Studies (CESAM) and Department of Biology, University of Aveiro, Campus Universitário Santiago, 3810-193, Aveiro, Portugal.
J C FrommletCentre for Environmental and Marine Studies (CESAM) and Department of Biology, University of Aveiro, Campus Universitário Santiago, 3810-193, Aveiro, Portugal.
A LouvadoCentre for Environmental and Marine Studies (CESAM) and Department of Biology, University of Aveiro, Campus Universitário Santiago, 3810-193, Aveiro, Portugal.
Y M HuangNational Penghu University of Science and Technology, Magong, Taiwan.
N J De VoogdNaturalis Biodiversity Center, Leiden, the Netherlands.
N C M GomesCentre for Environmental and Marine Studies (CESAM) and Department of Biology, University of Aveiro, Campus Universitário Santiago, 3810-193, Aveiro, Portugal. gomesncm@ua.pt.

Funding

Fundação para a Ciência e a Tecnologia UIDP/50017/2020+UIDB/50017/2020Horizon 2020 813360Ministry of Science and Technology, Taiwan MOST 110-2621-B-346-001Nederlandse Organisatie voor Wetenschappelijk Onderzoek 16.161.301
6 · The paper itself

Abstract

In the present study, we developed and validated an experimental life support system (ELSS) designed to investigate coral reef associated bacterial communities. The microcosms in the ELSS consisted of coral reef sediment, synthetic seawater, and specimens of five benthic reef species. These included two hard corals Montipora digitata and Montipora capricornis, a soft coral Sarcophyton glaucum, a zoanthid Zoanthus sp., and a sponge Chondrilla sp.. Physicochemical parameters and bacterial communities in the ELSS were similar to those observed at shallow coral reef sites. Sediment bacterial evenness and higher taxonomic composition were more similar to natural-type communities at days 29 and 34 than at day 8 after transfer to the microcosms, suggesting microbial stabilization after an initial recovery period. Biotopes were compositionally distinct but shared a number of ASVs. At day 34, sediment specific ASVs were found in hosts and visa versa. Transplantation significantly altered the bacterial community composition of M. digitata and Chondrilla sp., suggesting microbial adaptation to altered environmental conditions. Altogether, our results support the suitability of the ELSS developed in this study as a model system to investigate coral reef associated bacterial communities using multi-factorial experiments.

Indexed as

AnthozoaBacteriaCoral ReefsMicrobiotaAnimalsGeologic SedimentsLife Support SystemsSeawater

Identifiers

PMID39261551
PMCPMC11391067

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.