ArticleMicrobes and environments2024
Successful Isolation of Diverse Verrucomicrobiota Strains through the Duckweed-Microbes Co-cultivation Method.
Article in Microbes and environments, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Advances and Hotspots in Research on Verrucomicrobiota: Focus on Agroecosystems.Microbial ecology · 2025Review
- Complete genome sequence of a member of a potentially novel genus within the family Opitutaceae isolated from a liquid medium used for co-cultivating duckweeds and river water-derived microbes.Microbiology resource announcements · 2025Article
- Isolation of Ultra-SmallMicroorganisms · 2025Article
- Improvements in the Duckweed-Microbe Co-cultivation Method for the Stable and Efficient Isolation of Rarely Cultivated Bacteria Using Microfilter Membranes.Microbes and environments · 2025Article
- NovelFrontiers in microbiology · 2025Article
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9 authors.
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Abstract
The "duckweed-microbes co-cultivation method" is a microbial isolation technique that effectively recovers diverse microbes, including rarely cultivated bacterial phyla, from environmental samples. In this method, aseptic duckweed and microbes collected from an environmental sample are co-cultivated for several days, and duckweed-associated microbes are then isolated from its roots using a conventional agar plate-based cultivation method. We herein propose several improvements to the method in order to specifically obtain members of the rarely cultivated bacterial phylum, Verrucomicrobiota. In systems using river water as the inoculum, the marked enrichment of Verrucomicrobiota was observed after 10 days of co-cultivation, particularly in the roots and co-cultivated media. We also successfully isolated 44 strains belonging to subdivisions 1, 3, and 4 of the phylum Verrucomicrobiota from these systems. This was achieved by changing the concentration of nitrogen in the co-cultivation medium, which is known to affect duckweed growth and/or metabolism, and by subjecting the fronds and co-cultivated media as well as the roots after co-cultivation to microbial isolation.
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