Evidence map›Paper›PMID 39258780›Full record

ArticleBioTechniques2024

Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system.

Hiroyuki Morinaga, Yoh Sugawara, Yoshinori Kitagawa, Jingyuan Chen, Nobuo Yasuda, Hiroki Ogata, Yoshihiro Yamaguchi, Masao Kaneki, Joseph A Jeevendra Martyn, Shingo Yasuhara

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioTechniques, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Hiroyuki MorinagaDepartment of Anesthesiology, Critical Care & Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children, & Harvard Medical School.ORCID 0000-0003-3301-8154
Yoh SugawaraDepartment of Anesthesiology, Critical Care & Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children, & Harvard Medical School.
Yoshinori KitagawaDepartment of Anesthesiology, Critical Care & Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children, & Harvard Medical School.
Jingyuan ChenDepartment of Anesthesiology, Critical Care & Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children, & Harvard Medical School.
Nobuo YasudaOkayama Healthcare Professional University.
Hiroki OgataDepartment of Anesthesiology, Critical Care & Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children, & Harvard Medical School.
Yoshihiro YamaguchiDepartment of Trauma & Critical Care Medicine, Kyorin University,Faculty of Medicine.
Masao KanekiDepartment of Anesthesiology, Critical Care & Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children, & Harvard Medical School.
Joseph A Jeevendra MartynDepartment of Anesthesiology, Critical Care & Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children, & Harvard Medical School.
Shingo YasuharaDepartment of Anesthesiology, Critical Care & Pain Medicine, Massachusetts General Hospital, Shriners Hospitals for Children, & Harvard Medical School.

Funding

Major Burn Injury and its Effects on Acute and Superimposed Surgical PainR01GM142042 · NIGMS · MASSACHUSETTS GENERAL HOSPITAL · PI MARTYN, JEEVENDRA · 2020 to 2023
$1.3M
NIGMS NIH HHS R01 GM 142042NIGMS NIH HHS R01 GM142042Shriners Hospitals for Children 1158520, 158521, 85106, 86800
6 · The paper itself

Abstract

Modulating autophagy and mitophagy, vital cellular quality control systems, offer therapeutic potential for critical illnesses. However, limited drug screening options hinder progress. We present a novel assay using the photoswitchable fluorescent reporter, mito-Kaede, to quantify mitophagy flux. Mito-Kaede's superior UV-induced photoconversion and brightness post-conversion make it ideal for prolonged mitochondrial dynamics tracking. Its specificity in responding to mitophagy, confirmed by parkin-knockout cells, adds value. When coupled with a custom fluid exchange system, enabling efficient medium changes, precise mitophagy observations become feasible. This mitophagy assay, alongside our methodological insights, can decipher mitophagy's role in pathology and supports drug screening efforts.

Indexed as

MitophagyFluorescent DyesHeLa CellsHumansMitochondriaUbiquitin-Protein LigasesFluorescent Dyesparkin proteinUbiquitin-Protein LigasesassayautophagyCCCPfluorescent microscopymito-KaedemitophagyParkinphotoactivationphotoconversiontime-lapse

Identifiers

PMID39258780
PMCPMC12863277

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.