Evidence map›Paper›PMID 39257766›Full record

ArticlebioRxiv : the preprint server for biology2025

High-throughput synthesis and specificity characterization of natively paired antibodies using oPool

Wenhao O Ouyang, Huibin Lv, Wenkan Liu, Ruipeng Lei, Zongjun Mou, Tossapol Pholcharee, Logan Talmage, Meixuan Tong, Yiquan Wang, Katrine E Dailey and 6 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Wenhao O OuyangDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0002-3618-9808
Huibin LvDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0003-2218-0076
Wenkan LiuDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0009-0002-1702-3148
Ruipeng LeiDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0002-4652-3400
Zongjun MouDepartment of Physiology and Biophysics, Case Western Reserve University, School of Medicine, Cleveland, OH 44106, USA.ORCID 0000-0002-1908-617X
Tossapol PholchareeDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0002-5347-1936
Logan TalmageDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Meixuan TongDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Yiquan WangDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0002-1954-9808
Katrine E DaileyDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Akshita B GopalDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0002-6429-0073
Danbi ChoiDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Madison R ArdaghDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Lucia A RodriguezDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Xinghong DaiDepartment of Physiology and Biophysics, Case Western Reserve University, School of Medicine, Cleveland, OH 44106, USA.ORCID 0000-0002-4102-3175
Nicholas C WuDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0002-9078-6697

Funding

Sequence-function relationship of influenza broadly neutralizing antibodiesR01AI167910 · NIAID · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI Nicholas C. Wu · 2022 to 2026
$2.5M
High-throughput identification of antibody features for sequence-based epitope predictionDP2AT011966 · NCCIH · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI WU, NICHOLAS C. · 2021 to 2024
$2.4M
NCCIH NIH HHS DP2 AT011966NIAID NIH HHS R01 AI167910
6 · The paper itself

Abstract

Antibody discovery is crucial for developing therapeutics and vaccines as well as understanding adaptive immunity. However, the lack of approaches to synthesize antibodies with defined sequences in a high-throughput manner represents a major bottleneck in antibody discovery. Here, we presented oPool

Indexed as

antibodyB-cell receptorsbroadly neutralizingcryo-EMhemagglutininHigh-throughput screeninginfluenzamRNA displayoligo pools

Identifiers

PMID39257766
PMCPMC11383711

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.