Evidence map›Paper›PMID 39257749›Full record

ArticlebioRxiv : the preprint server for biology2024

Computational design of serine hydrolases.

Anna Lauko, Samuel J Pellock, Ivan Anischanka, Kiera H Sumida, David Juergens, Woody Ahern, Alex Shida, Andrew Hunt, Indrek Kalvet, Christoffer Norn and 8 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

18 authors.

Anna LaukoDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID 0000-0001-5903-3518
Samuel J PellockDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID 0000-0002-7557-7985
Ivan AnischankaDepartment of Biochemistry, University of Washington, Seattle, WA, USA.
Kiera H SumidaDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID 0000-0003-2773-9676
David JuergensDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID 0000-0001-6425-8391
Woody AhernDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID 0009-0006-1247-8847
Alex ShidaDepartment of Biochemistry, University of Washington, Seattle, WA, USA.
Andrew HuntDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID 0000-0001-9620-593X
Indrek KalvetDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID 0000-0002-6610-2857
Christoffer NornDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID 0000-0002-1450-4651
Ian R HumphreysDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID 0000-0002-3058-6714
Cooper JamiesonDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, USA.ORCID 0000-0002-6076-6230
Alex KangDepartment of Biochemistry, University of Washington, Seattle, WA, USA.
Evans BrackenbroughDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID 0009-0004-1476-0219
Asim K BeraDepartment of Biochemistry, University of Washington, Seattle, WA, USA.
Banumathi SankaranDepartment of Biochemistry, University of Washington, Seattle, WA, USA.
K N HoukDepartment of Chemistry and Biochemistry, University of California, Los Angeles, California, USA.
David BakerDepartment of Biochemistry, University of Washington, Seattle, WA, USA.ORCID 0000-0003-3645-2044

Funding

User Training and OutreachP30GM124169 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI PAUL David ADAMS, Gregory L Hura · 2017 to 2026
$28.6M
TRAINING IN MOLECULAR BIOPHYSICST32GM008268 · NIGMS · UNIVERSITY OF WASHINGTON · PI KOLLMAN, JUSTIN M, ZHENG, NING · 1988 to 2023
$8.6M
NIGMS NIH HHS P30 GM124169NIGMS NIH HHS T32 GM008268
6 · The paper itself

Abstract

Enzymes that proceed through multistep reaction mechanisms often utilize complex, polar active sites positioned with sub-angstrom precision to mediate distinct chemical steps, which makes their de novo construction extremely challenging. We sought to overcome this challenge using the classic catalytic triad and oxyanion hole of serine hydrolases as a model system. We used RFdiffusion

Identifiers

PMID39257749
PMCPMC11384011

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.