ArticlePlant, cell & environment2025
Spatial and Temporal Freezing Dynamics of Leaves Revealed by Time-Lapse Imaging.
Article in Plant, cell & environment, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Molecular mechanisms of plant freezing tolerance: from cold signal perception to adaptive responses.Frontiers in plant science · 2026Review
- The cold embrace of death: using time-lapse imaging to understand what kills leaves when they freeze.Journal of experimental botany · 2025Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Freezing air temperatures kill most leaves, yet the leaves of some species can survive these events. Tracking the temporal and spatial dynamics of freezing remains an impediment to characterizing frost tolerance. Here we deploye time-lapse imaging and image subtraction analysis, coupled with fine wire thermocouples, to discern the in situ spatial dynamics of freezing and thawing. Our method of analysis of pixel brightness reveals that ice formation in leaves exposed to natural frosts initiates in mesophyll before spreading to veins, and that while ex situ xylem sap freezes near 0°C, in situ xylem sap has a freezing point of -2°C in our model freezing-resistant species of Lonicera. Photosynthetic rates in leaves that have been exposed to a rapid freeze or thaw do not recover, but leaves exposed to a slow, natural freezing and thawing to -10°C do recover. Using this method, we are able to quantify the spatial formation and timing of freezing events in leaves, and suggest that in situ and ex situ freezing points for xylem sap can differ by more than 4°C depending on the rate of temperature decline.
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Registered trials
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