ArticleDevelopment (Cambridge, England)2024
Tissue-specific RNA-seq defines genes governing male tail tip morphogenesis in C. elegans.
Article in Development (Cambridge, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Developmental constraints in the repeated evolution of male tail characters in rhabditid and diplogastrid nematodes.PloS one · 2026Article
- Article
- Morphologically defined substages of tail morphogenesis in C. elegans males.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Review
- Morphologically defined substages of tail morphogenesis inbioRxiv : the preprint server for biology · 2024Article
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Abstract
Caenorhabditis elegans males undergo sex-specific tail tip morphogenesis (TTM) under the control of the DM-domain transcription factor DMD-3. To find genes regulated by DMD-3, we performed RNA-seq of laser-dissected tail tips. We identified 564 genes differentially expressed (DE) in wild-type males versus dmd-3(-) males and hermaphrodites. The transcription profile of dmd-3(-) tail tips is similar to that in hermaphrodites. For validation, we analyzed transcriptional reporters for 49 genes and found male-specific or male-biased expression for 26 genes. Only 11 DE genes overlapped with genes found in a previous RNAi screen for defective TTM. GO enrichment analysis of DE genes finds upregulation of genes within the unfolded protein response pathway and downregulation of genes involved in cuticle maintenance. Of the DE genes, 40 are transcription factors, indicating that the gene network downstream of DMD-3 is complex and potentially modular. We propose modules of genes that act together in TTM and are co-regulated by DMD-3, among them the chondroitin synthesis pathway and the hypertonic stress response.
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