Evidence map›Paper›PMID 39252136›Full record

ArticleBiological research2024

Mouse testicular macrophages can independently produce testosterone and are regulated by Cebpb.

Nengliang Duan, Yuanshuai Ran, Huapei Wang, Ya Luo, Zhixiang Gao, Xingyu Lu, Fengmei Cui, Qiu Chen, Boxin Xue, Xiaolong Liu

Abstract read
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In one paragraph

Article in Biological research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Nengliang DuanDepartment of Urology, The Second Affiliated Hospital of Soochow University, NO.1055 SanXiang Road, Suzhou, Jiangsu Province, 215000, China.ORCID https://orcid.org/0000-0002-1161-1996
Yuanshuai RanDepartment of Urology, The Second Affiliated Hospital of Soochow University, NO.1055 SanXiang Road, Suzhou, Jiangsu Province, 215000, China.
Huapei WangDepartment of Urology, The Second Affiliated Hospital of Soochow University, NO.1055 SanXiang Road, Suzhou, Jiangsu Province, 215000, China.
Ya LuoDepartment of Urology, The Second Affiliated Hospital of Soochow University, NO.1055 SanXiang Road, Suzhou, Jiangsu Province, 215000, China.
Zhixiang GaoDepartment of Urology, The Second Affiliated Hospital of Soochow University, NO.1055 SanXiang Road, Suzhou, Jiangsu Province, 215000, China.
Xingyu LuState Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China.
Fengmei CuiState Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China. cuifengmei@suda.edu.cn.
Qiu ChenState Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China. happyqiu@suda.edu.cn.
Boxin XueDepartment of Urology, The Second Affiliated Hospital of Soochow University, NO.1055 SanXiang Road, Suzhou, Jiangsu Province, 215000, China. xueboxin1992@suda.edu.cn.
Xiaolong LiuDepartment of Urology, The Second Affiliated Hospital of Soochow University, NO.1055 SanXiang Road, Suzhou, Jiangsu Province, 215000, China. liuxiaolong2005@suda.edu.cn.ORCID http://orcid.org/0009-0004-5457-885X

Funding

Jiangsu Province Occupational Health Research Project JSZJ20233201Occupational health research project in Jiangsu Province No. JSZJ20233201Postgraduate Research & Practice Innovation Program of Jiangsu Province KYCX24_3345Provincial and Ministerial Co-constructed Open Topics for the State Key Laboratory of Radiological Medicine and Radiation Protection GZK12023038Suzhou City Basic Research Program - Key Clinical Technology Research SKY2023004Suzhou City Key Core Technology Breakthrough - Social Development Project 2023SS19Suzhou Medical College Joint Project by Four Parties ML12301223Suzhou Science and Technology Bureau - Key clinical technology No. SKY2023004Youth Excellence Projects of CNNC CNNC-2020Youth Excellence Projects of Suzhou Health No. GSWS2020031
6 · The paper itself

Abstract

backgroundTesticular macrophages (TM) have long been recognized for their role in immune response within the testicular environment. However, their involvement in steroid hormone synthesis, particularly testosterone, has not been fully elucidated. This study aims to explore the capability of TM to synthesize and secrete testosterone de novo and to investigate the regulatory mechanisms involved.

resultsTranscriptomic analysis revealed significant expression of Cyp11a1, Cyp17a1, Hsd3b1, and Hsd17b3 in TM, which are key enzymes in the testosterone synthesis pathway. qPCR analysis and immunofluorescence validation confirmed the autonomous capability of TM to synthesize testosterone. Ablation of TM in mice resulted in decreased physiological testosterone levels, underscoring the significance of TM in maintaining testicular testosterone levels. Additionally, the study also demonstrated that Cebpb regulates the expression of these crucial genes, thereby modulating testosterone synthesis.

conclusionsThis research establishes that TM possess the autonomous capacity to synthesize and secrete testosterone, contributing significantly to testicular testosterone levels. The transcription factor Cebpb plays a crucial role in this process by regulating the expression of key genes involved in testosterone synthesis.

Indexed as

CCAAT-Enhancer-Binding Protein-betaMacrophagesTestisTestosteroneAnimalsGene Expression RegulationMaleTranscriptomeCCAAT-Enhancer-Binding Protein-betaTestosteroneCebpbDe novo synthesisTesticular macrophageTestosterone

Identifiers

PMID39252136
PMCPMC11382419

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.