Bipolar electrochemical growth of conductive microwires for cancer spheroid integration: a step forward in conductive biological circuitry.

The field of bioelectronics is developing exponentially. There is now a drive to interface electronics with biology for the development of new technologies to improve our understanding of electrical forces in biology. This builds on our recently published work in which we show wireless electrochemistry could be used to grow bioelectronic functional circuitry in 2D cell layers. To date our ability to merge electronics with in situ with biology is 3D limited. In this study, we aimed to further develop the wireless electrochemical approach for the self-assembly of microwires in situ with custom-designed and fabricated 3D cancer spheroids. Unlike traditional electrochemical methods that rely on direct electrical connections to induce currents, our technique utilises bipolar electrodes that operate independently of physical wired connections. These electrodes enable redox reactions through the application of an external electric field. Specifically, feeder electrodes connected to a power supply generate an electric field, while the bipolar electrodes, not physically connected to the feeder electrodes, facilitate the reduction of silver ions from the solution. This process occurs upon applying a voltage across the feeder electrodes, resulting in the formation of self-assembled microwires between the cancer spheroids.Thereby, creating interlinked bioelectronic circuitry with cancer spheroids. We demonstrate that a direct current was needed to stimulate the growth of conductive microwires in the presence of cell spheroids. Microwire growth was successful when using 50 V (0.5 kV/cm) of DC applied to a single spheroid of approximately 800 µm in diameter but could not be achieved with alternating currents. This represents the first proof of the concept of using wireless electrochemistry to grow conductive structures with 3D mammalian cell spheroids.