ArticleNature communications2024
Label-free 3D molecular imaging of living tissues using Raman spectral projection tomography.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Confocal Raman Microscopy Quantitatively Reveals Subcellular Metabolic Adaptation Mechanisms of Deep-Sea Mussels to Methane Deprivation.Environmental science & technology · 2026Article
- Label-free molecular profiling of cancer using Raman spectroscopy: from fundamentals to clinical applications.Frontiers in oncology · 2026Review
- Label-free linear and non-linear vibrational spectroscopy for functional materials: state-of-the-art and future perspectives.Chemical science · 2025Review
- A new paradigm for tumour profiling: Spatiotemporal omics in living tissue.Clinical and translational medicine · 2025Article
- Spectrally Silent and Optically Transparent: Clear-SiR for Deep Raman Biomolecular Sensing.ACS sensors · 2025Article
- Novel Multimodal Imaging System for High-Resolution and High-Contrast Tissue Segmentation Based on Chemical Properties.Sensors (Basel, Switzerland) · 2025Article
- Label-free optical microscopy with artificial intelligence: a new paradigm in pathology.Biophotonics discovery · 2025Review
- Multiparametric physicochemical analysis of a type 1 collagen 3D cell culture model using light and electron microscopy and mass spectrometry imaging.Scientific reports · 2025Article
- Surface-Enhanced Raman Spectroscopy for Biomedical Applications: Recent Advances and Future Challenges.ACS applied materials & interfaces · 2025Review
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
The ability to image tissues in three dimensions (3D) with label-free molecular contrast at the mesoscale would be a valuable capability in biology and biomedicine. Here, we introduce Raman spectral projection tomography (RSPT) for volumetric molecular imaging with optical sub-millimeter spatial resolution. We have developed a RSPT imaging instrument capable of providing 3D molecular contrast in transparent and semi-transparent samples. We also created a computational pipeline for multivariate reconstruction to extract label-free spatial molecular information from Raman projection data. Using these tools, we demonstrate imaging and visualization of phantoms of various complex shapes with label-free molecular contrast. Finally, we apply RSPT as a tool for imaging of molecular gradients and extracellular matrix heterogeneities in fixed and living tissue-engineered constructs and explanted native cartilage tissues. We show that there exists a favorable balance wherein employing Raman spectroscopy, with its advantages in live cell imaging and label-free molecular contrast, outweighs the reduction in imaging resolution and blurring caused by diffuse photon propagation. Thus, RSPT imaging opens new possibilities for label-free molecular monitoring of tissues.
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Registered trials
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