Evidence map›Paper›PMID 39251530›Full record

ArticlePlanta2024

Genome-wide identification of oxidosqualene cyclase genes regulating natural rubber in Taraxacum kok-saghyz.

Yongfei Wang, Zhanchao Yang, Boxuan Yuan, Lixia He, Yunyi Han, Juanying Wang, Xuchu Wang

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yongfei WangKey Laboratory of Tropical Islands Ecology, Ministry of Education, College of Life Sciences, Hainan Normal University, Haikou, 571158, People's Republic of China.
Zhanchao YangKey Laboratory of Tropical Islands Ecology, Ministry of Education, College of Life Sciences, Hainan Normal University, Haikou, 571158, People's Republic of China.
Boxuan YuanKey Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, College of Life Sciences, Guizhou University, Guiyang, 550025, People's Republic of China.
Lixia HeKey Laboratory of Tropical Islands Ecology, Ministry of Education, College of Life Sciences, Hainan Normal University, Haikou, 571158, People's Republic of China.
Yunyi HanKey Laboratory of Tropical Islands Ecology, Ministry of Education, College of Life Sciences, Hainan Normal University, Haikou, 571158, People's Republic of China.
Juanying WangKey Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education, College of Life Sciences, Guizhou University, Guiyang, 550025, People's Republic of China.
Xuchu WangKey Laboratory of Tropical Islands Ecology, Ministry of Education, College of Life Sciences, Hainan Normal University, Haikou, 571158, People's Republic of China. xcwang@gzu.edu.cn.ORCID http://orcid.org/0000-0003-3137-4390

Funding

the Innovation Platform for Academicians of Hainan Province 2022YSCXTD0005the National Key Research and Development Program of China No. 2021YFA1300401the Special Fund for Talents of Guizhou University NO. LJ2023-03
6 · The paper itself

Abstract

MAIN

conclusionNine TkOSC genes have been identified by genome-wide screening. Among them, TkOSC4-6 might be more crucial for natural rubber biosynthesis in Taraxacum kok-saghyz roots. Taraxacum kok-saghyz Rodin (TKS) roots contain large amounts of natural rubber, inulin, and valuable metabolites. Oxidosqualene cyclase (OSC) is a key member for regulating natural rubber biosynthesis (NRB) via the triterpenoid biosynthesis pathway. To explore the functions of OSC on natural rubber producing in TKS, its gene family members were identified in TKS genome via genome-wide screening. Nine TkOSCs were identified, which were mainly distributed in the cytoplasm. Their family genes experienced a neutral selection during the evolution process. Overall sequence homology analysis OSC proteins revealed 80.23% similarity, indicating a highly degree of conservation. Pairwise comparisons showed a multiple sequence similarity ranging from 57% to 100%. Protein interaction prediction revealed that TkOSCs may interact with baruol synthase, sterol 1,4-demethylase, lupeol synthase and squalene epoxidase. Phylogenetic analysis showed that OSC family proteins belong to two branches. TkOSC promoter regions contain cis-acting elements related to plant growth, stress response, hormones response and light response. Protein accumulation analysis demonstrated that TkOSC4, TkOSC5 and TkOSC6 proteins had strong expression levels in the root, latex and plumular axis. Comparison of gene expression patterns showed TkOSC1, TkOSC4, TkOSC5, TkOSC6, TkOSC7, TkOSC8 and TkOSC9 might be important in regulating NRB. Combination of gene and protein results revealed TkOSC4-6 might be more crucial, and the data might contribute to a more profound understanding of the roles of OSCs for NRB in TKS roots.

Indexed as

Gene Expression Regulation, PlantIntramolecular TransferasesPhylogenyRubberTaraxacumGenome, PlantPlant ProteinsPlant RootsIntramolecular Transferaseslanosterol synthasePlant ProteinsRubberGene expressionGene family analysisNatural rubber biosynthesisOxidosqualene cyclaseTaraxacum kok-saghyz

Identifiers

PMID39251530

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.