Evidence map›Paper›PMID 39250531›Full record

ArticleDevelopment (Cambridge, England)2024

miR-31-mediated local translation at the mitotic spindle is important for early development.

Carolyn M Remsburg, Kalin D Konrad, Michael D Testa, Nadezda Stepicheva, Kelvin Lee, Leila H Choe, Shawn Polson, Jaysheel Bhavsar, Hongzhan Huang, Jia L Song

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Carolyn M RemsburgDepartment of Biological Sciences, University of Delaware, Newark, DE 19716, USA.ORCID 0000-0002-8977-9334
Kalin D KonradDepartment of Biological Sciences, University of Delaware, Newark, DE 19716, USA.ORCID 0009-0000-5405-7967
Michael D TestaDepartment of Biological Sciences, University of Delaware, Newark, DE 19716, USA.
Nadezda StepichevaDepartment of Biological Sciences, University of Delaware, Newark, DE 19716, USA.
Kelvin LeeDepartment of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA.ORCID 0000-0003-0908-2981
Leila H ChoeDepartment of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA.
Shawn PolsonDepartment of Computer and Informational Sciences; Plant & Soil Sciences; Biological Sciences, CBCB Bioinformatics Core Facility; Bioinformatics, Healthcare Informatics, and Data Science Network of Delaware, University of Delaware, Newark, DE 19716, USA.ORCID 0000-0002-3398-6932
Jaysheel BhavsarDepartment of Computer and Informational Sciences, University of Delaware, DE 19716, USA.
Hongzhan HuangDepartment of Computer and Informational Sciences, University of Delaware, DE 19716, USA.
Jia L SongDepartment of Biological Sciences, University of Delaware, Newark, DE 19716, USA.ORCID 0000-0002-5664-1136

Funding

Predictive Modeling & Optimal Control Framework for Model-Based Epidemic Response in DelawareP20GM103446 · NIGMS · UNIVERSITY OF DELAWARE · PI Shawn W Polson · 2012 to 2026
$67.2M
Updated confocal microscope in the Imaging Core facility to support biomedical researchP20GM103653 · NIGMS · DELAWARE STATE UNIVERSITY · PI HARRINGTON, MELISSA A · 2012 to 2022
$22.7M
Vivarium CoreP30GM145765 · NIGMS · DELAWARE STATE UNIVERSITY · PI MELISSA A HARRINGTON · 2022 to 2026
$7.7M
Delaware IDeA Network of Biomedical Research Excellence P20GM103446National Science Foundation IOS 1553338NIGMS NIH HHS P20 GM103446NIGMS NIH HHS P20 GM103653NIGMS NIH HHS P30 GM145765NIH HHS P20GM103653Sigma Xi 20A01418
6 · The paper itself

Abstract

miR-31 is a highly conserved microRNA that plays crucial roles in cell proliferation, migration and differentiation. We discovered that miR-31 and some of its validated targets are enriched on the mitotic spindle of the dividing sea urchin embryo and mammalian cells. Using the sea urchin embryo, we found that miR-31 inhibition led to developmental delay correlated with increased cytoskeletal and chromosomal defects. We identified miR-31 to directly suppress several actin remodeling transcripts, including β-actin, Gelsolin, Rab35 and Fascin. De novo translation of Fascin occurs at the mitotic spindle of sea urchin embryos and mammalian cells. Importantly, miR-31 inhibition leads to a significant a increase of newly translated Fascin at the spindle of dividing sea urchin embryos. Forced ectopic localization of Fascin transcripts to the cell membrane and translation led to significant developmental and chromosomal segregation defects, highlighting the importance of the regulation of local translation by miR-31 at the mitotic spindle to ensure proper cell division. Furthermore, miR-31-mediated post-transcriptional regulation at the mitotic spindle may be an evolutionarily conserved regulatory paradigm of mitosis.

Indexed as

MicroRNAsProtein BiosynthesisSpindle ApparatusActinsAnimalsCarrier ProteinsChromosome SegregationEmbryonic DevelopmentEmbryo, NonmammalianGene Expression Regulation, DevelopmentalHumansMicrofilament ProteinsMitosisSea UrchinsActinsCarrier ProteinsMicrofilament ProteinsMicroRNAsCell divisionCleavage stageCytoskeletal elementsSea urchin

Identifiers

PMID39250531
PMCPMC11423917

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.