Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Richard Quintana-Feliciano *Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.ORCID 0000-0002-5835-4683
Jithesh Kottur *Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA. jitheshkottur@iav.res.in.
Mi NiDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.ORCID 0000-0002-8930-6887
Rikhia GhoshDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Leslie Salas-EstradaDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Goran AhlsenDepartment of Biochemistry and Molecular Biophysics Columbia University Vagelos College of Physicians and Surgeons, New York, NY, USA.
Olga RechkoblitDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.ORCID 0000-0003-4270-7242
Lawrence ShapiroDepartment of Biochemistry and Molecular Biophysics Columbia University Vagelos College of Physicians and Surgeons, New York, NY, USA.
Marta FilizolaDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.ORCID 0000-0002-4382-8276
Gang FangDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.ORCID 0000-0002-2462-9124
Aneel K AggarwalDepartment of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA. aneel.aggarwal@mssm.edu.ORCID 0000-0003-4389-0173
Funding
Conduits: Mount Sinai Health System Translational Science HubUL1TR004419 · NCATS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Rosalind J Wright · 2022 to 2026
$46.4M
X-ray Scattering Technology CoreP30GM133893 · NIGMS · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI Sean McSweeney · 2019 to 2026
$38.6M
High Resolution Characterization of Bacterial Epigenomes and MicrobiomeR35GM139655 · NIGMS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Gang Fang · 2021 to 2026
$4.9M
Structure and Specificity of Restriction-Modification (R-M) SystemsR35GM131780 · NIGMS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI ANEEL K. AGGARWAL · 2019 to 2026
$3.4M
COVID and Translational Science supercomputer (CATS)S10OD030463 · OD · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI KOVATCH, PATRICIA · 2021 to 2021
$2.0M
Big Omics Data Engine 2 SupercomputerS10OD026880 · OD · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI KOVATCH, PATRICIA · 2019 to 2019
$2.0M
Department of Energy DE-SC0012704NCATS NIH HHS UL1 TR004419NIGMS NIH HHS P30 GM133893NIGMS NIH HHS R35 GM131780NIGMS NIH HHS R35 GM139655NIH HHS S10 OD026880NIH HHS S10 OD030463U.S. Department of Health & Human Services | National Institutes of Health (NIH) R35-GM131780U.S. Department of Health & Human Services | National Institutes of Health (NIH) R35GM13965
6 · The paper itself
Abstract
Burkholderia cenocepacia is an opportunistic and infective bacterium containing an orphan DNA methyltransferase called M.BceJIV with roles in regulating gene expression and motility of the bacterium. M.BceJIV recognizes a GTWWAC motif (where W can be an adenine or a thymine) and methylates N6 of the adenine at the fifth base position. Here, we present crystal structures of M.BceJIV/DNA/sinefungin ternary complex and allied biochemical, computational, and thermodynamic analyses. Remarkably, the structures show not one, but two DNA substrates bound to the M.BceJIV dimer, with each monomer contributing to the recognition of two recognition sequences. We also show that methylation at the two recognition sequences occurs independently, and that the GTWWAC motifs are enriched in intergenic regions in the genomes of B. cenocepacia strains. We further computationally assess the interactions underlying the affinities of different ligands (SAM, SAH, and sinefungin) for M.BceJIV, as a step towards developing selective inhibitors for limiting B. cenocepacia infection.
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
Burkholderia cenocepacia epigenetic regulator M.BceJIV simultaneously engages two DNA recognition sequences for methylation. · full record | OpenQuestion