Evidence map›Paper›PMID 39242832›Full record

ArticleNature methods2024

Simultaneous multicolor fluorescence imaging using PSF splitting.

Robin Van den Eynde, Fabian Hertel, Sergey Abakumov, Bartosz Krajnik, Siewert Hugelier, Alexander Auer, Joschka Hellmeier, Thomas Schlichthaerle, Rachel M Grattan, Diane S Lidke and 4 more

Abstract read
In one paragraph

Article in Nature methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Robin Van den EyndeDepartment of Chemistry, KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0001-6548-3312
Fabian HertelDepartment of Chemistry, KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0003-2825-1138
Sergey AbakumovDepartment of Chemistry, KU Leuven, Leuven, Belgium.ORCID http://orcid.org/0000-0002-3834-5966
Bartosz KrajnikDepartment of Chemistry, KU Leuven, Leuven, Belgium.
Siewert HugelierDepartment of Chemistry, KU Leuven, Leuven, Belgium.
Alexander AuerMax Planck Institute of Biochemistry, Martinsried, Germany.ORCID http://orcid.org/0000-0002-4969-8767
Joschka HellmeierMax Planck Institute of Biochemistry, Martinsried, Germany.ORCID http://orcid.org/0000-0002-7153-9121
Thomas SchlichthaerleMax Planck Institute of Biochemistry, Martinsried, Germany.
Rachel M GrattanDepartment of Pathology and Comprehensive Cancer Center, University of New Mexico, Albuquerque, NM, USA.ORCID http://orcid.org/0000-0003-1204-6615
Diane S LidkeDepartment of Pathology and Comprehensive Cancer Center, University of New Mexico, Albuquerque, NM, USA.
Ralf JungmannMax Planck Institute of Biochemistry, Martinsried, Germany.ORCID http://orcid.org/0000-0003-4607-3312
Marcel LeuteneggerMax Planck Institute of Biophysical Chemistry, Göttingen, Germany.
Wim VandenbergDepartment of Chemistry, KU Leuven, Leuven, Belgium. wim.vandenberg@kuleuven.be.ORCID http://orcid.org/0000-0002-5888-9100
Peter DedeckerDepartment of Chemistry, KU Leuven, Leuven, Belgium. peter.dedecker@kuleuven.be.ORCID http://orcid.org/0000-0002-1882-2075

Funding

Imaging the Early Events in Membrane Receptor SignalingR35GM126934 · NIGMS · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI Diane Lidke · 2018 to 2026
$3.1M
Fonds Wetenschappelijk Onderzoek (Research Foundation Flanders) G010723NFonds Wetenschappelijk Onderzoek (Research Foundation Flanders) G090819NNIGMS NIH HHS R35 GM126934U.S. Department of Health & Human Services | National Institutes of Health (NIH) R35GM126934
6 · The paper itself

Abstract

We present a way to encode more information in fluorescence imaging by splitting the original point spread function (PSF), which offers broadband operation and compatibility with other PSF engineering modalities and existing analysis tools. We demonstrate the approach using the 'Circulator', an add-on that encodes the fluorophore emission band into the PSF, enabling simultaneous multicolor super-resolution and single-molecule microscopy using essentially the full field of view.

Indexed as

Microscopy, FluorescenceAlgorithmsFluorescent DyesHumansImage Processing, Computer-AssistedOptical ImagingSingle Molecule ImagingFluorescent Dyes

Identifiers

PMID39242832
PMCPMC12558347

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.