Evidence map›Paper›PMID 39239997›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2024

Liquid Phase Electron Microscopy of Bacterial Ultrastructure.

Brian J Caffrey, Adrián Pedrazo-Tardajos, Emanuela Liberti, Benjamin Gaunt, Judy S Kim, Angus I Kirkland

Abstract read
In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Formation Mechanism of BN Flakes on MWCNTs.Small (Weinheim an der Bergstrasse, Germany) · 2026
    Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. Liquid Phase Electron Microscopy of Bacterial Ultrastructure.Small (Weinheim an der Bergstrasse, Germany) · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Brian J CaffreyThe Rosalind Franklin Institute, Harwell Science and Innovation Campus, Didcot, OX11 OQX, UK.ORCID 0000-0001-5040-4247
Adrián Pedrazo-TardajosThe Rosalind Franklin Institute, Harwell Science and Innovation Campus, Didcot, OX11 OQX, UK.ORCID 0000-0003-4181-9226
Emanuela LibertiThe Rosalind Franklin Institute, Harwell Science and Innovation Campus, Didcot, OX11 OQX, UK.ORCID 0000-0001-5306-7936
Benjamin GauntThe Rosalind Franklin Institute, Harwell Science and Innovation Campus, Didcot, OX11 OQX, UK.ORCID 0000-0002-3071-7227
Judy S KimThe Rosalind Franklin Institute, Harwell Science and Innovation Campus, Didcot, OX11 OQX, UK.ORCID 0000-0003-1513-9964
Angus I KirklandThe Rosalind Franklin Institute, Harwell Science and Innovation Campus, Didcot, OX11 OQX, UK.ORCID 0000-0001-8068-1990

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recent advances in liquid phase scanning transmission electron microscopy (LP-STEM) have enabled the study of dynamic biological processes at nanometer resolutions, paving the way for live-cell imaging using electron microscopy. However, this technique is often hampered by the inherent thickness of whole cell samples and damage from electron beam irradiation. These restrictions degrade image quality and resolution, impeding biological interpretation. Using graphene encapsulation, scanning transmission electron microscopy (STEM), and energy-dispersive X-ray (EDX) spectroscopy to mitigate these issues provides unprecedented levels of intracellular detail in aqueous specimens. This study demonstrates the potential of LP-STEM to examine and identify internal cellular structures in thick biological samples. Specifically, it highlights the use of LP-STEM to investigate the radiation resistant, gram-positive bacterium, Deinococcus radiodurans using various imaging techniques.

Indexed as

DeinococcusMicroscopy, Electron, Scanning TransmissionBacteriaGraphiteSpectrometry, X-Ray EmissionGraphitedeinococcus.radioduransenergy dispersive x‐ray spectroscopygraphene encapsulationliquid phasemanganese uptakeradiation resistancescanning transmission electron microscopy

Identifiers

PMID39239997
PMCPMC11636060

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.