Evidence map›Paper›PMID 39238099›Full record

ArticleThe journal of trauma and acute care surgery2024

Platelet-rich plasma enhances rib fracture strength and callus formation in vivo.

Adrian Camarena, Lillian Kang, Anthony J Mirando, Emily Augustine, Najerie S McMillian, Natasha C Stinson, Suresh M Agarwal, Matthew L Becker, Matthew J Hilton, Joseph S Fernandez-Moure

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Article in The journal of trauma and acute care surgery, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Adrian CamarenaFrom the Department of Surgery (A.C., L.K., S.M.A., J.S.F.-M.), Duke University Medical Center; Department of Orthopedic Surgery (A.J.M., M.J.H.), Duke University School of Medicine; Division of Trauma, Acute, and Critical Care Surgery (N.S.M., S.M.A., J.S.F.-M.), Duke University Medical Center; and Department of Chemistry (E.A., N.C.S., M.L.B.), Department of Mechanical Engineering and Materials Science (E.A., N.C.S., M.L.B.), Department of Biomedical Engineering (E.A., N.C.S., M.L.B.), Department of Orthopedic Surgery (E.A., N.C.S., M.L.B.), and Department of Cell Biology (M.J.H.), Duke University, Durham, North Carolina.ORCID 0000-0001-8512-0607
Lillian Kang
Anthony J Mirando
Emily Augustine
Najerie S McMillian
Natasha C Stinson
Suresh M Agarwal
Matthew L Becker
Matthew J Hilton
Joseph S Fernandez-Moure

Funding

Resorbable, Phsophorylated Poly(ester urea) Surgical Adhesive to Enhance Fracture HealingK08AR079609 · NIAMS · DUKE UNIVERSITY · PI FERNANDEZ-MOURE, JOSEPH S. · 2021 to 2025
$835k
NIAMS NIH HHS K08 AR079609NIAMS NIH HHS L30 AR083193
6 · The paper itself

Abstract

backgroundRib fractures are a common traumatic injury affecting more than 350,000 patients a year. Early stabilization has shown to be effective in reducing pulmonary complications. Platelet-rich plasma (PRP) is a growth factor-rich blood product known to improve soft tissue and bone healing. We hypothesized that the addition of PRP to a rib fracture site would accelerate callus formation and improve callus strength.

methodsPlatelet-rich plasma was isolated from pooled Lewis rat blood and quantified. Thirty-two Lewis rats underwent fracture of the sixth rib and were treated with 100 μL PRP (1 × 10 6 platelets/μL) or saline. At 2 weeks, ribs were harvested and underwent a 3-point bend, x-ray, and microcomputed tomography, and callus sections were stained with 4',6-diamidino-2-phenylindole and Alcian blue and picrosirius red. At 6 weeks, ribs were harvested and underwent a 3-point bend test, x-ray, microcomputed tomography, and Alcian blue and picrosirius red staining.

resultsAt 2 weeks, PRP increased callus diameter (9.3 mm vs. 4.3 mm, p = 0.0002), callus index (4.5 vs. 2.1, p = 0.0002), bone volume/total volume (0.0551 vs. 0.0361, p = 0.0024), cellularization (2,364 vs. 1,196, p < 0.0001), and cartilage (12.12% vs. 3.11%, p = 0.0001) and collagen (6.64% vs. 4.85%, p = 0.0087) content compared with controls. At 6 weeks, PRP increased fracture callus diameter (5.0 mm vs. 4.0 mm, 0.0466), callus index (2.5 vs. 2.0, p = 0.0466), BV/TV (0.0415 vs. 0.0308, p = 0.0358), and higher cartilage (8.21% vs. 3.26%, p < 0.0001) and collagen (37.61% vs. 28.00%, p = 0.0022) content compared with controls. At 6 weeks, PRP samples trended toward improved mechanical characteristics; however, these results did not reach significance ( p > 0.05).

conclusionRib fractures are a common injury, and accelerated stabilization could improve clinical outcomes. Platelet-rich plasma significantly increased callus size, calcium deposition, and cartilage and collagen content at 2 and 6 weeks and trended toward improved strength and toughness on mechanical analysis at 6 weeks compared with controls, although this did not reach significance. These findings suggest that PRP may be a useful adjunct to accelerate and improve fracture healing in high-risk patients.

Indexed as

Bony CallusFracture HealingPlatelet-Rich PlasmaRib FracturesAnimalsDisease Models, AnimalMaleRatsRats, Inbred LewX-Ray Microtomographyfracture repairPlatelet-rich plasmaratsrib fractures

Identifiers

PMID39238099
PMCPMC12396507

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.