Evidence map›Paper›PMID 39236692›Full record

ArticleOncology2025

A Novel Index Including Age, Sex, hTERT, and Methylated RUNX3 Is Useful for Diagnosing Early Gastric Cancer.

Katsuhiko Nakamura, Yutaka Suehiro, Koichi Hamabe, Atsushi Goto, Shinichi Hashimoto, Yuki Kunimune, Akiyo Ishiguro, Naoko Okayama, Tomohiro Fujii, Yukiko Nakahara and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Katsuhiko NakamuraDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Yutaka SuehiroDepartment of Oncology and Laboratory Medicine, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Koichi HamabeDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Atsushi GotoDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Shinichi HashimotoDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Yuki KunimuneDepartment of Oncology and Laboratory Medicine, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Akiyo IshiguroDepartment of Oncology and Laboratory Medicine, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Naoko OkayamaDivision of Medical Genetics, Yamaguchi University Hospital, Ube, Japan.
Tomohiro FujiiDivision of Laboratory, Yamaguchi University Hospital, Ube, Japan.
Yukiko NakaharaDivision of Laboratory, Yamaguchi University Hospital, Ube, Japan.
Mitsuaki NishiokaDivision of Laboratory, Yamaguchi University Hospital, Ube, Japan.
Shingo HigakiDepartment of Gastroenterology, St. Hill Hospital, Ube, Japan.
Ikuei FujiiDepartment of Health Screening, Ajisu Kyoritsu Hospital, Yamaguchi, Japan.
Chieko SuzukiDepartment of Internal Medicine, Ajisu Kyoritsu Hospital, Yamaguchi, Japan.
Jun NishikawaFaculty of Laboratory Science, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Isao SakaidaDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Taro TakamiDepartment of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, Ube, Japan.
Takahiro YamasakiDepartment of Oncology and Laboratory Medicine, Yamaguchi University Graduate School of Medicine, Ube, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionAs the incidence of gastric cancer (GC) is increasing in East Asia including Japan, a simple blood test for early GC is needed as an alternative to upper gastrointestinal (UGI) endoscopy. We performed this study to address this issue.

methodsWe collected serum samples from 319 participants comprising 225 healthy subjects without GC (control group) and 94 patients with early GC (early GC group). After evaluating copy numbers of serum hTERT and methylated RUNX3 (m-RUNX3) using the Combined Restriction Digital PCR (CORD) assay, which we developed, we assessed the diagnostic performance of hTERT and m-RUNX3 for early GC.

resultsSerum levels of hTERT and m-RUNX3 were significantly higher in the early GC group than in the control group. The area under the curve (AUC) was 0.89 for hTERT and 0.78 for m-RUNX3. Multivariate logistic regression analysis revealed age, sex, hTERT copy number, and m-RUNX3 copy number to be independent factors for early GC. We then established a prediction formula and named it the ASTEm-R3 (age, sex, hTERT, and m-RUNX3) index. The AUC of the ASTEm-R3 index was 0.93 with a sensitivity of 79.7% and specificity of 91.1%.

conclusionWe demonstrated excellent performance of the ASTEm-R3 index using the CORD assay to detect early GC. This index might be a promising alternative to UGI endoscopy.

introductionAs the incidence of gastric cancer (GC) is increasing in East Asia including Japan, a simple blood test for early GC is needed as an alternative to upper gastrointestinal (UGI) endoscopy. We performed this study to address this issue.

methodsWe collected serum samples from 319 participants comprising 225 healthy subjects without GC (control group) and 94 patients with early GC (early GC group). After evaluating copy numbers of serum hTERT and methylated RUNX3 (m-RUNX3) using the Combined Restriction Digital PCR (CORD) assay, which we developed, we assessed the diagnostic performance of hTERT and m-RUNX3 for early GC.

resultsSerum levels of hTERT and m-RUNX3 were significantly higher in the early GC group than in the control group. The area under the curve (AUC) was 0.89 for hTERT and 0.78 for m-RUNX3. Multivariate logistic regression analysis revealed age, sex, hTERT copy number, and m-RUNX3 copy number to be independent factors for early GC. We then established a prediction formula and named it the ASTEm-R3 (age, sex, hTERT, and m-RUNX3) index. The AUC of the ASTEm-R3 index was 0.93 with a sensitivity of 79.7% and specificity of 91.1%.

conclusionWe demonstrated excellent performance of the ASTEm-R3 index using the CORD assay to detect early GC. This index might be a promising alternative to UGI endoscopy.

Indexed as

Biomarkers, TumorCore Binding Factor Alpha 3 SubunitStomach NeoplasmsTelomeraseAdultAgedAge FactorsCase-Control StudiesDNA MethylationEarly Detection of CancerFemaleHumansMaleMiddle AgedSex FactorsBiomarkers, TumorCore Binding Factor Alpha 3 SubunitRunx3 protein, humanTelomeraseTERT protein, humanBiomarkerEarly detectionGastric cancerLiquid biopsy

Identifiers

PMID39236692
PMCPMC11965857

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.