Observational studyPLoS neglected tropical diseases2024
Diagnosis of brucellosis: Combining tests to improve performance.
Observational study in PLoS neglected tropical diseases, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Beyond Serology: A Meta-Analysis of Advancements in Molecular Detection of Brucella spp. in Seronegative Animals and Biological Samples.Veterinary medicine and science · 2025Pooled it
- Development of a Visual RPA-CRISPR/Cas12a Assay TargetingVeterinary sciences · 2026Article
- High brucellosis prevalence and risk among children aged 5-14 years in Kenyan pastoral health facilities: A facility-based surveillance study.PLoS neglected tropical diseases · 2026Article
- Bacterial Granulomatous Lung Diseases: Radiological Findings and Differential Diagnosis.Infectious disease reports · 2026Review
- Detection of Bovine Brucellosis Antibodies in Serum and Milk Using Quantum Dot Microspheres Immunochromatographic Assay.Microorganisms · 2026Article
- Brucellosis Seroprevalence, Analysis of Risk Factors, and Comparison of Test Methods Used in Diagnosis in a Tertiary Hospital in Kahramanmaraş.Tropical medicine and infectious disease · 2026Article
- Rapid serological detection of smoothFrontiers in cellular and infection microbiology · 2026Article
- Prevalence and detection ofFrontiers in cellular and infection microbiology · 2026Article
- Stratum-specific serum metabolic reprogramming in Saanen goats with graded Brucella serological reactivity under natural exposure.Frontiers in veterinary science · 2026Article
- Congenital brucellosis in a preterm infant: A case report from France and literature review.IDCases · 2026Article
- Optimizing ovine brucellosis serodiagnosis: evaluation of recombinantFrontiers in veterinary science · 2026Article
- Development and evaluation of novel Brucella diagnostic antigen protein ST.BMC infectious diseases · 2025Article
- Serological diagnosis of human brucellosis in Morocco and prospects for advanced diagnostic techniques.Iranian journal of microbiology · 2025Article
- Development of a Chemiluminescence Immunoassay for the Serological Diagnosis of Sheep and Bovine Brucellosis.Microorganisms · 2025Article
- [Evaluation of Gradient-Variant Quantum Dot Fluorescence Technology for Rapid Detection ofSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2025Article
- Molecular detection, seroprevalence, and phylogenetic analysis ofVeterinary world · 2025Article
- Application of Brucella type IV secretion proteins VirB1, VirB5, and VirB6 in the serological diagnosis of brucellosis.Scientific reports · 2025Article
- Preparation of a Brucella multiepitope fusion protein based on bioinformatics and its application in serological diagnosis of human brucellosis.Scientific reports · 2025Article
- Brucella-associated hemophagocytic syndrome: case report of a potentially life-threatening condition and literature review.Frontiers in immunology · 2025Review
- Unraveling brucellosis: advances in pathogenesis, diagnostic strategies, therapeutic innovations, and public health perspectives.Frontiers in medicine · 2025Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionBrucellosis, a zoonotic infectious disease caused by bacteria of the genus Brucella, remains a significant global health concern in many parts of the world. Traditional diagnostic methods, including serological tests, suffer from limitations, including low sensibility and high false-positive rates, emphasizing the need for improved diagnostic strategies. In this study, we aimed to optimize diagnostic accuracy by reevaluating serological tests and exploring novel diagnostic algorithms.
methodsA retrospective observational study was conducted using sera collected between June 2012 and June 2023 at the French National Reference Center for Brucella. Various serological tests, including Rose Bengal plate test (RBT), standard agglutination test (SAT), Brucellacapt, and ELISA for IgM and IgG, were performed. Different diagnostic algorithms were evaluated, combining RBT with SAT, Brucellacapt, and ELISA to enhance the performance of diagnostic tests.
resultsAmong 3587 sera analyzed, 148 were confirmed cases of human brucellosis. Individual serological tests exhibited good sensitivity and specificity but lacked diagnostic accuracy. However, combining RBT with SAT or Brucellacapt significantly improved diagnostic performance, with reduced false positives. The most promising results were observed when an algorithm was built combining RBT, Brucellacapt, and ELISA for IgM and IgG (a score value of 0.5 with 90.5% for sensitivity, 99.7% for specificity, 92.4% for PPV, and 99.6% for NPV).
conclusionsSerological tests remain crucial for brucellosis diagnosis, but their limitations necessitate innovative diagnostic approaches. Combining multiple serological tests in diagnostic algorithms shows promise in improving diagnostic accuracy. Efforts to refine diagnostic, strengthen surveillance, and raise awareness are essential for effective brucellosis control, particularly in resource-limited settings.
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