Evidence map›Paper›PMID 39235750›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Illumina MiSeq 16S rRNA Gene Library Preparation for Poultry Processing Microbiome Analyses.

Jessica A Brown, Kristina M Feye, Steven C Ricke

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jessica A BrownMeat Science and Animal Biologics Discovery, Department of Animal & Dairy Sciences, University of Wisconsin-Madison, Madison, WI, USA.
Kristina M FeyeCell and Molecular Biology, University of Arkansas, Fayetteville, AR, USA.
Steven C RickeMeat Science and Animal Biologics Discovery, Department of Animal & Dairy Sciences, University of Wisconsin-Madison, Madison, WI, USA. sricke@wisc.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The standardization of the microbiome sequencing of poultry rinsates is essential for generating comparable microbial composition data among poultry processing facilities if this technology is to be adopted by the industry. Samples must first be acquired, DNA must be extracted, and libraries must be constructed. In order to proceed to library sequencing, the samples should meet quality control standards. Finally, data must be analyzed using computer bioinformatics pipelines. This data can subsequently be incorporated into more advanced computer algorithms for risk assessment. Ultimately, *a uniform sequencing pipeline will enable both the government regulatory agencies and the poultry industry to identify potential weaknesses in food safety.This chapter presents the different steps for monitoring the population dynamics of the microbiome in poultry processing using 16S rDNA sequencing.

Indexed as

Gene LibraryHigh-Throughput Nucleotide SequencingMicrobiotaPoultryRNA, Ribosomal, 16SAnimalsComputational BiologyDNA, BacterialSequence Analysis, DNADNA, BacterialRNA, Ribosomal, 16S16S rRNA geneIlluminaMicrobiomeMOTHURPoultry processingQIIME2Sequencing

Identifiers

PMID39235750

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.