ArticleMethods in molecular biology (Clifton, N.J.)2025
The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Development and validation of a quantitative pcr array assay for the detection of viral sequences in breast cancer.Journal, genetic engineering & biotechnology · 2026Article
- Review
- Loop-Mediated Isothermal Amplification-Based Workflow for the Detection and Serotyping ofFoods (Basel, Switzerland) · 2024Article
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Authors and funding
4 authors.
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Abstract
Foodborne pathogens continue to be a major health concern worldwide. Culture-dependent methodologies are still considered the gold standard to perform pathogen detection and quantification. These methods present several drawbacks, such as being time-consuming and labor intensive. The implementation of real-time PCR has allowed to overcome these limitations, and even reduce the cost associated with the analyses, due to the possibility of simultaneously and accurately detecting several pathogens in one single assay, with results comparable to those obtained by classical approaches. In this chapter, a protocol for the simultaneous detection of two of the most important foodborne pathogens, Salmonella spp. and Listeria monocytogenes, is described.
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